Confirmation of optimal excitation and emission wavelengths based on indicator kriging method

Excitation wavelength and emission wavelength are vital parameters of molecular fluorescence spectral measurement, whose choice influences the precision of molecular fluorescence spectral measurement and its subsequent study. Because measurement precision of some spectral instrument such as fluorescence spectrometer HITACHI F-4500 can not reach the requirement of spectral analytical technique, and the measurement data is finite, in practical work, the required data are obtained from measurement data using interpolation, which becomes an effective solving method. Therefore, the present paper studies the confirmation of certain volume percentage ethanol solution's optimal excitation wavelength and optimal emission wavelength using indicator Kriging method: the optimal excitation wavelength and optimal emission wavelength based on indicator Kriging method are 226.9 and 337.1 nm respectively, with verification. Furthermore, the interpolation result is valuated using relative standard deviation. These results indicate that it is feasible to confirm the optimal excitation wavelength and optimal emission wavelength based on indicator Kriging method in the molecular fluorescence spectral measurement, which supplies a novel method for the confirmation of optimal excitation wavelength and optimal emission wavelength.