Colorimetric determination of cysteine based on Au@Pt nanoparticles as oxidase mimetics with enhanced selectivity

A H₂O₂-free colorimetric protocol based on urchin-like Au@Pt nanoparticles (Au@Pt NPs) has been developed for the sensitive and selective determination of cysteine (Cys). We verified the intrinsic oxidase-like activity of the Au@Pt NPs. They can act as artificial mimic oxidases to catalyse the oxidization of 3,3′,5,5′-tetramethylbenzidine (TMB) with the assistance of dissolved oxygen, avoiding the use of H₂O₂ in the colorimetric determination of Cys. In addition, the discrimination of Cys from the other two biothiol analogues, homocysteine and glutathione, can be easily realized through a simple ageing process. HNO₃ is added to this colorimetric system to terminate the reaction by oxidizing ox-TMB (oxidized form of TMB) to diphenoquinone (DPQ), thus generating a characteristic absorption peak of DPQ at 450 nm. By recording the absorbance at 450 nm, interference from the aggregated Au@Pt NPs (absorption peak at 670 nm) when 650 nm (the characteristic absorption peak of ox-TMB) is used as the absorption wavelength can be eliminated. We investigated this H₂O₂-free colorimetric protocol and obtained high sensitivity, with a detection limit of 1.5 nM and relatively high selectivity. The analytical performance for real samples was further explored. The Au@Pt NP–based H₂O₂-free colorimetric protocol is of great significance for the sensitive and selective determination of Cys in practical samples in different scenarios. Graphical abstract: [Figure not available: see fulltext.].