Cloning of the full-length cDNA sequence of a novel human UCA1 spliced variant

Objective: To clone the full-length cDNA sequence of novel UCA1 spliced isoforms for understanding the exact mechanism of this type of alternative splicing. Methods: The full-length cDNA was amplified from BLZ-211 cells by using the in silicon sequence elongation technique, 5'-RACE and 3'-RACE techniques. Products of RT-PCR were sequenced and further assembled. Results: The new UCA1 spliced isoform sequence was 2202 bp. Conclusion: A combination of the in silicon sequence elongation, 5'-RACE and 3'-RACE techniques is an effective way to obtain the full-length cDNA, which will guide further research on the mechanism of this type of alternative splicing.