Cloning of New Antigen Gene MLAA-34 Promoter and Identification of Core Region in Acute Monocytic Leukemia

Bo Lei; Wang Gang Zhang; Ai Li He; Yin Xia Chen; Xing Mei Cao; Wan Hong Zhao; Jian Li Wang; Jie Liu; Xiao Rong Ma; Peng Yu Zhang; Ju Bai

doi:10.19746/j.cnki.issn.1009-2137.2019.03.002

Cloning of New Antigen Gene MLAA-34 Promoter and Identification of Core Region in Acute Monocytic Leukemia

Bo Lei
, Wang Gang Zhang
, Ai Li He
, Yin Xia Chen
, Xing Mei Cao
, Wan Hong Zhao
, Jian Li Wang
, Jie Liu
, Xiao Rong Ma
, Peng Yu Zhang
, Ju Bai

Xi'an Jiaotong University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

题目: 急性单核细胞白血病新抗原基因MLAA-34启动子的克隆及其核心区鉴定. 目的: 克隆急性单核细胞白血病新抗原基因MLAA-34上游启动子序列并检测其活性、鉴定核心区域. 方法: PCR扩增 MLAA-34基因启动子区全长片段,将其连接至pGL3Basic 载体，克隆重组质粒; 构建一系列 MLAA-34基因启动子 5′侧翼区截短质粒; 将含MLAA-34启动子序列的重组质粒及其截短型质粒转染至U937、HEK293细胞，应用双荧光素酶报告基因检测各片段的启动子活性，确定启动子最小活性区域，并通过生物信息学方法分析转录因子结合位点. 结果: 构建了含有MLAA-34启动子序列的重组质粒及其截短型质粒；与空载体相比，其启动子活性明显增加(P＜0.001) MLAA-34最小活性区域位于转录起始位点－402 bp至－200 bp之间，其中包含E2F1，MZF-1，SP1，USF2和STAT3等多个转录因子结合位点. 结论: 成功构建急性单核细胞白血病新抗原基因MLAA-34不同缺失片段的启动子荧光素酶报告基因重组质粒，鉴定出核心启动子区，其中含有多个潜在的转录因子结合序列.

Original language	English
Pages (from-to)	641-645
Number of pages	5
Journal	Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology
Volume	27
Issue number	3
DOIs	https://doi.org/10.19746/j.cnki.issn.1009-2137.2019.03.002
State	Published - 1 Jun 2019

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10.19746/j.cnki.issn.1009-2137.2019.03.002

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Lei, B., Zhang, W. G., He, A. L., Chen, Y. X., Cao, X. M., Zhao, W. H., Wang, J. L., Liu, J., Ma, X. R., Zhang, P. Y., & Bai, J. (2019). Cloning of New Antigen Gene MLAA-34 Promoter and Identification of Core Region in Acute Monocytic Leukemia. Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology, 27(3), 641-645. https://doi.org/10.19746/j.cnki.issn.1009-2137.2019.03.002

@article{3afb1656652e410da8e61e7eae3306bf,

title = "Cloning of New Antigen Gene MLAA-34 Promoter and Identification of Core Region in Acute Monocytic Leukemia",

abstract = "题目: 急性单核细胞白血病新抗原基因MLAA-34启动子的克隆及其核心区鉴定. 目的: 克隆急性单核细胞白血病新抗原基因MLAA-34上游启动子序列并检测其活性、鉴定核心区域. 方法: PCR扩增 MLAA-34基因启动子区全长片段,将其连接至pGL3Basic 载体，克隆重组质粒; 构建一系列 MLAA-34基因启动子 5′侧翼区截短质粒; 将含MLAA-34启动子序列的重组质粒及其截短型质粒转染至U937、HEK293细胞，应用双荧光素酶报告基因检测各片段的启动子活性，确定启动子最小活性区域，并通过生物信息学方法分析转录因子结合位点. 结果: 构建了含有MLAA-34启动子序列的重组质粒及其截短型质粒；与空载体相比，其启动子活性明显增加(P＜0.001) MLAA-34最小活性区域位于转录起始位点－402 bp至－200 bp之间，其中包含E2F1，MZF-1，SP1，USF2和STAT3等多个转录因子结合位点. 结论: 成功构建急性单核细胞白血病新抗原基因MLAA-34不同缺失片段的启动子荧光素酶报告基因重组质粒，鉴定出核心启动子区，其中含有多个潜在的转录因子结合序列.",

author = "Bo Lei and Zhang, \{Wang Gang\} and He, \{Ai Li\} and Chen, \{Yin Xia\} and Cao, \{Xing Mei\} and Zhao, \{Wan Hong\} and Wang, \{Jian Li\} and Jie Liu and Ma, \{Xiao Rong\} and Zhang, \{Peng Yu\} and Ju Bai",

year = "2019",

month = jun,

day = "1",

doi = "10.19746/j.cnki.issn.1009-2137.2019.03.002",

language = "英语",

volume = "27",

pages = "641--645",

journal = "Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology",

issn = "1009-2137",

publisher = "Haidian-qu",

number = "3",

}

Lei, B, Zhang, WG, He, AL, Chen, YX, Cao, XM, Zhao, WH, Wang, JL, Liu, J, Ma, XR, Zhang, PY & Bai, J 2019, 'Cloning of New Antigen Gene MLAA-34 Promoter and Identification of Core Region in Acute Monocytic Leukemia', Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology, vol. 27, no. 3, pp. 641-645. https://doi.org/10.19746/j.cnki.issn.1009-2137.2019.03.002

Cloning of New Antigen Gene MLAA-34 Promoter and Identification of Core Region in Acute Monocytic Leukemia. / Lei, Bo; Zhang, Wang Gang; He, Ai Li et al.
In: Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology, Vol. 27, No. 3, 01.06.2019, p. 641-645.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Cloning of New Antigen Gene MLAA-34 Promoter and Identification of Core Region in Acute Monocytic Leukemia

AU - Lei, Bo

AU - Zhang, Wang Gang

AU - He, Ai Li

AU - Chen, Yin Xia

AU - Cao, Xing Mei

AU - Zhao, Wan Hong

AU - Wang, Jian Li

AU - Liu, Jie

AU - Ma, Xiao Rong

AU - Zhang, Peng Yu

AU - Bai, Ju

PY - 2019/6/1

Y1 - 2019/6/1

N2 - 题目: 急性单核细胞白血病新抗原基因MLAA-34启动子的克隆及其核心区鉴定. 目的: 克隆急性单核细胞白血病新抗原基因MLAA-34上游启动子序列并检测其活性、鉴定核心区域. 方法: PCR扩增 MLAA-34基因启动子区全长片段,将其连接至pGL3Basic 载体，克隆重组质粒; 构建一系列 MLAA-34基因启动子 5′侧翼区截短质粒; 将含MLAA-34启动子序列的重组质粒及其截短型质粒转染至U937、HEK293细胞，应用双荧光素酶报告基因检测各片段的启动子活性，确定启动子最小活性区域，并通过生物信息学方法分析转录因子结合位点. 结果: 构建了含有MLAA-34启动子序列的重组质粒及其截短型质粒；与空载体相比，其启动子活性明显增加(P＜0.001) MLAA-34最小活性区域位于转录起始位点－402 bp至－200 bp之间，其中包含E2F1，MZF-1，SP1，USF2和STAT3等多个转录因子结合位点. 结论: 成功构建急性单核细胞白血病新抗原基因MLAA-34不同缺失片段的启动子荧光素酶报告基因重组质粒，鉴定出核心启动子区，其中含有多个潜在的转录因子结合序列.

AB - 题目: 急性单核细胞白血病新抗原基因MLAA-34启动子的克隆及其核心区鉴定. 目的: 克隆急性单核细胞白血病新抗原基因MLAA-34上游启动子序列并检测其活性、鉴定核心区域. 方法: PCR扩增 MLAA-34基因启动子区全长片段,将其连接至pGL3Basic 载体，克隆重组质粒; 构建一系列 MLAA-34基因启动子 5′侧翼区截短质粒; 将含MLAA-34启动子序列的重组质粒及其截短型质粒转染至U937、HEK293细胞，应用双荧光素酶报告基因检测各片段的启动子活性，确定启动子最小活性区域，并通过生物信息学方法分析转录因子结合位点. 结果: 构建了含有MLAA-34启动子序列的重组质粒及其截短型质粒；与空载体相比，其启动子活性明显增加(P＜0.001) MLAA-34最小活性区域位于转录起始位点－402 bp至－200 bp之间，其中包含E2F1，MZF-1，SP1，USF2和STAT3等多个转录因子结合位点. 结论: 成功构建急性单核细胞白血病新抗原基因MLAA-34不同缺失片段的启动子荧光素酶报告基因重组质粒，鉴定出核心启动子区，其中含有多个潜在的转录因子结合序列.

UR - https://www.scopus.com/pages/publications/85068237839

U2 - 10.19746/j.cnki.issn.1009-2137.2019.03.002

DO - 10.19746/j.cnki.issn.1009-2137.2019.03.002

M3 - 文章

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AN - SCOPUS:85068237839

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JO - Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology

JF - Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology

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Lei B, Zhang WG, He AL, Chen YX, Cao XM, Zhao WH et al. Cloning of New Antigen Gene MLAA-34 Promoter and Identification of Core Region in Acute Monocytic Leukemia. Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology. 2019 Jun 1;27(3):641-645. doi: 10.19746/j.cnki.issn.1009-2137.2019.03.002

Cloning of New Antigen Gene MLAA-34 Promoter and Identification of Core Region in Acute Monocytic Leukemia

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