Chronic stress inhibits testosterone synthesis in Leydig cells through mitochondrial damage via Atp5a1

Xiaofan Xiong; Qiuhua Wu; Lingyu Zhang; Shanfeng Gao; Rufeng Li; Lin Han; Meiyang Fan; Miaomiao Wang; Liying Liu; Xiaofei Wang; Chunli Zhang; Yanlong Xin; Zongfang Li; Chen Huang; Juan Yang

doi:10.1111/jcmm.17085

Chronic stress inhibits testosterone synthesis in Leydig cells through mitochondrial damage via Atp5a1

Xiaofan Xiong
, Qiuhua Wu
, Lingyu Zhang
, Shanfeng Gao
, Rufeng Li
, Lin Han
, Meiyang Fan
, Miaomiao Wang
, Liying Liu
, Xiaofei Wang
, Chunli Zhang
, Yanlong Xin
, Zongfang Li
, Chen Huang
, Juan Yang

Health Science Center

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Stress is one of the leading causes of male infertility, but its exact function in testosterone synthesis has scarcely been reported. We found that adult male rats show a decrease in bodyweight, genital index and serum testosterone level after continual chronic stress for 21 days. Two-dimensional gel electrophoresis (2-DE) and MALDI-TOF-MS analysis identified 10 differentially expressed proteins in stressed rats compared with controls. A strong protein interaction network was found to be centred on Atp5a1 among these proteins. Atp5a1 expression significantly decreased in Leydig cells after chronic stress. Transfection of Atp5a1 siRNAs decreased StAR, CYP11A1, and 17β-HSD expression by damaging the structure of mitochondria in TM3 cells. This study confirmed that chronic stress plays an important role in testosterone synthesis by regulating Atp5a1 expression in Leydig cells.

Original language	English
Pages (from-to)	354-363
Number of pages	10
Journal	Journal of Cellular and Molecular Medicine
Volume	26
Issue number	2
DOIs	https://doi.org/10.1111/jcmm.17085
State	Published - Jan 2022

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10.1111/jcmm.17085

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title = "Chronic stress inhibits testosterone synthesis in Leydig cells through mitochondrial damage via Atp5a1",

abstract = "Stress is one of the leading causes of male infertility, but its exact function in testosterone synthesis has scarcely been reported. We found that adult male rats show a decrease in bodyweight, genital index and serum testosterone level after continual chronic stress for 21 days. Two-dimensional gel electrophoresis (2-DE) and MALDI-TOF-MS analysis identified 10 differentially expressed proteins in stressed rats compared with controls. A strong protein interaction network was found to be centred on Atp5a1 among these proteins. Atp5a1 expression significantly decreased in Leydig cells after chronic stress. Transfection of Atp5a1 siRNAs decreased StAR, CYP11A1, and 17β-HSD expression by damaging the structure of mitochondria in TM3 cells. This study confirmed that chronic stress plays an important role in testosterone synthesis by regulating Atp5a1 expression in Leydig cells.",

author = "Xiaofan Xiong and Qiuhua Wu and Lingyu Zhang and Shanfeng Gao and Rufeng Li and Lin Han and Meiyang Fan and Miaomiao Wang and Liying Liu and Xiaofei Wang and Chunli Zhang and Yanlong Xin and Zongfang Li and Chen Huang and Juan Yang",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley \& Sons Ltd.",

year = "2022",

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doi = "10.1111/jcmm.17085",

language = "英语",

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TY - JOUR

T1 - Chronic stress inhibits testosterone synthesis in Leydig cells through mitochondrial damage via Atp5a1

AU - Xiong, Xiaofan

AU - Wu, Qiuhua

AU - Zhang, Lingyu

AU - Gao, Shanfeng

AU - Li, Rufeng

AU - Han, Lin

AU - Fan, Meiyang

AU - Wang, Miaomiao

AU - Liu, Liying

AU - Wang, Xiaofei

AU - Zhang, Chunli

AU - Xin, Yanlong

AU - Li, Zongfang

AU - Huang, Chen

AU - Yang, Juan

PY - 2022/1

Y1 - 2022/1

N2 - Stress is one of the leading causes of male infertility, but its exact function in testosterone synthesis has scarcely been reported. We found that adult male rats show a decrease in bodyweight, genital index and serum testosterone level after continual chronic stress for 21 days. Two-dimensional gel electrophoresis (2-DE) and MALDI-TOF-MS analysis identified 10 differentially expressed proteins in stressed rats compared with controls. A strong protein interaction network was found to be centred on Atp5a1 among these proteins. Atp5a1 expression significantly decreased in Leydig cells after chronic stress. Transfection of Atp5a1 siRNAs decreased StAR, CYP11A1, and 17β-HSD expression by damaging the structure of mitochondria in TM3 cells. This study confirmed that chronic stress plays an important role in testosterone synthesis by regulating Atp5a1 expression in Leydig cells.

AB - Stress is one of the leading causes of male infertility, but its exact function in testosterone synthesis has scarcely been reported. We found that adult male rats show a decrease in bodyweight, genital index and serum testosterone level after continual chronic stress for 21 days. Two-dimensional gel electrophoresis (2-DE) and MALDI-TOF-MS analysis identified 10 differentially expressed proteins in stressed rats compared with controls. A strong protein interaction network was found to be centred on Atp5a1 among these proteins. Atp5a1 expression significantly decreased in Leydig cells after chronic stress. Transfection of Atp5a1 siRNAs decreased StAR, CYP11A1, and 17β-HSD expression by damaging the structure of mitochondria in TM3 cells. This study confirmed that chronic stress plays an important role in testosterone synthesis by regulating Atp5a1 expression in Leydig cells.

UR - https://www.scopus.com/pages/publications/85120870109

U2 - 10.1111/jcmm.17085

DO - 10.1111/jcmm.17085

M3 - 文章

C2 - 34894202

AN - SCOPUS:85120870109

SN - 1582-1838

VL - 26

SP - 354

EP - 363

JO - Journal of Cellular and Molecular Medicine

JF - Journal of Cellular and Molecular Medicine

IS - 2

ER -

Chronic stress inhibits testosterone synthesis in Leydig cells through mitochondrial damage via Atp5a1

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