TY - JOUR
T1 - Cell proliferation in ependymal/subventricular zone and nNOS expression following focal cerebral ischemia in adult rats
AU - Zhang, Pengbo
AU - Liu, Yong
AU - Li, Jie
AU - Kang, Qianyan
AU - Tian, Yingfang
AU - Chen, Xinlin
AU - Shi, Qindong
AU - Song, Tusheng
PY - 2006/1
Y1 - 2006/1
N2 - Neuronal nitric oxide synthase (nNOS) regulates neurogenesis in normal developing brain, but the role of nNOS in neurogenesis in the ischemic brain remains unclear. To investigate the temporal and spatial relationship between cell proliferation of the ependymal/subventricular zone (SVZ), a principal neuroproliferative region in the adult brain, and nNOS expression, the male Sprague-Dawley rats weighing 250-350 g were used. The focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO). 10 μl of 0.2% fluorescence dye Dil was injected into the right lateral ventricle to prelabel ependymal/subventricular zone cells before ischemia. The rats were killed immediately after iscnemia and days 1, 3, 7, 11, 14, 21 and 28 after ischemia. Oil-labeled cell counting was employed to assess cell proliferation. Immunohistochemistry and grayscale analysis were performed to determine nNOS localization and its quantity in the specific regions. Compared with control, the density of Dil-labeled cells in the ipsilateral ependyma/SVZ was significantly higher at days 1, 3, 7 and 11 after ischemia, whereas the quantity of nNOS expression in the ependyma/SVZ adjacent regions was significantly lower at the above time points. Additionally, nNOS positive cells were largely excluded from SVZ, and their long processes did not enter the ependyma/SVZ. Our results indicate that after focal cerebral ischemia, decreased nNOS expression in the ipsilateral ependymal/SVZ adjacent regions might be related to cell proliferation in the ependymal/SVZ.
AB - Neuronal nitric oxide synthase (nNOS) regulates neurogenesis in normal developing brain, but the role of nNOS in neurogenesis in the ischemic brain remains unclear. To investigate the temporal and spatial relationship between cell proliferation of the ependymal/subventricular zone (SVZ), a principal neuroproliferative region in the adult brain, and nNOS expression, the male Sprague-Dawley rats weighing 250-350 g were used. The focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO). 10 μl of 0.2% fluorescence dye Dil was injected into the right lateral ventricle to prelabel ependymal/subventricular zone cells before ischemia. The rats were killed immediately after iscnemia and days 1, 3, 7, 11, 14, 21 and 28 after ischemia. Oil-labeled cell counting was employed to assess cell proliferation. Immunohistochemistry and grayscale analysis were performed to determine nNOS localization and its quantity in the specific regions. Compared with control, the density of Dil-labeled cells in the ipsilateral ependyma/SVZ was significantly higher at days 1, 3, 7 and 11 after ischemia, whereas the quantity of nNOS expression in the ependyma/SVZ adjacent regions was significantly lower at the above time points. Additionally, nNOS positive cells were largely excluded from SVZ, and their long processes did not enter the ependyma/SVZ. Our results indicate that after focal cerebral ischemia, decreased nNOS expression in the ipsilateral ependymal/SVZ adjacent regions might be related to cell proliferation in the ependymal/SVZ.
KW - Focal cerebral ischemia
KW - Neurogenesis
KW - Neuronal nitric oxide synthase
KW - Rat
UR - https://www.scopus.com/pages/publications/32044438254
U2 - 10.1179/016164106X91942
DO - 10.1179/016164106X91942
M3 - 文章
C2 - 16464370
AN - SCOPUS:32044438254
SN - 0161-6412
VL - 28
SP - 91
EP - 96
JO - Neurological Research
JF - Neurological Research
IS - 1
ER -