Cell behaviors on polysaccharide-wrapped single-wall carbon nanotubes: A quantitative study of the surface properties of biomimetic nanofibrous scaffolds

Natural polysaccharides such as amylose (AMY), alginate sodium (ALG), and chitosan (CHI) have been noncovalently wrapped onto single-wall carbon nanotubes (SWCNTs) to give a series of SWCNT scaffolds, termed as AMY-SWCNT, ALG-SWCNT, CHI-SWCNT, and CHI/ALG-SWCNT scaffolds. Compared to purified SWCNTs and oxidized SWCNTs, the polysaccharide-wrapped SWCNTs can well mimic nanofibrous extracellular matrix and significantly enhance cell adhesion and proliferation. The surface properties of the SWCNT scaffolds, such as functional groups, surface charge, and hydrophilicity, can all directly influence the protein adsorption and lead to changes in cellular FAK expression, thus affect the mammalian cell morphology and proliferation. By quantitatively studying the surface properties of these SWCNT scaffolds, it can be concluded that relatively positively charged hydrophilic scaffolds that bear -OH groups can remarkably promote cell growth. Considering all properties, the relatively electrical neutral and hydrophilic AMY-SWCNT scaffolds bearing only -OH groups are able to sustain the highest cell viability after 72 h culturing.