CD133+干细胞在胰腺癌放疗耐受中的作用

Objective： To study the role of pancreatic cancer stem cells in radiotherapy resistance of pancreatic cancer. Methods： CD133+ pancreatic cancer stem cells were obtained by using magnetic cell sorting. MTT and flow cytometry were used to observe the proliferation activity and apoptosis of different cell subsets of pancreatic cancer after radiation treatment. Then flow cytometry, Western blot and qRT-PCR were used to observe the expression changes of CD133 in pancreatic cancer cells after radiation treatment. Finally, the cell cycle distribution of different subgroup cells was observed by flow cytometry. Results： Compared with unselected cells and CD133- cells, CD133+ stem cells had the highest survival rate and the lowest apoptosis rate after radiation treatment. The proportion of CD133+ stem cells increased and the expressions of CD133 protein and RNA increased in pancreatic cancer cells. CD133+ stem cells had the highest ratio of G₀/G₁ phase compared with unsorted cells and CD133- cells, and the difference was statistically significant. Conclusion： CD133+ pancreatic cancer stem cells are one of the important causes of radiotherapy resistance in pancreatic cancer cells, and the increased proportion of G₀/G₁ phase may be one of the mechanisms.