Cation exchange resin-induced hydrolysis for improving biodegradability of waste activated sludge: Characterization of dissolved organic matters and microbial community

This study reported an efficient and green approach towards facilitating hydrolysis of waste activated sludge (WAS) using cation exchange resin (CER) as a recyclable additive. Through CER-mediated removal of multivalent cations, WAS flocs were disintegrated into small particles with extracellular polymeric substance (EPS) solubilization. At CER dosage of 1.75 g/g SS, SCOD increased to 2579 mg/L (SCOD/TCOD = 15.9%) after 8-h hydrolysis. Afterwards, CER displayed further sludge hydrolysis performance lasting 2 days, i.e. SCOD/TCOD = 34.2%. Meanwhile, proteins, carbohydrates and other organics in dissolved organic matters (DOMs) were major contributors for volatile fatty acids (VFAs) accumulation, with composition percentage: VFAs (58.9%) > proteins (21.8%) > other organics (8.8%) > humic acids (5.9%) > carbohydrates (4.4%). The biodegradable tryptophan-like and tyrosine-like proteins were major proteins, while other organics included amino acids, aliphatic and metabolic intermediates. More than 85.2% of DOMs were easily biodegradable. Moreover, CER-induced hydrolysis modified microbial community structure through inhibiting VFAs-utilizing microbes, while hydrolytic-acidogenic bacteria were enriched, responsible for DOMs biodegradation.