Calcite yield for bacteria induced precipitation

Bacteria precipitation is a method for producing calcium carbonate. An important parameter for characterizing bacteria precipitation is the yield of calcite precipitated per unit volume of solution. A series of orthogonal experiments were performed to investigate the influence of culture concentration, substrate concentration, the inoculation quantity of bacteria and the crystal formation additive on the precipitation amount and the calcite yield. The results show that the precipitation amount and the calcite yield are mainly dictated by the substrate concentration. When the culture concentration, the inoculation quantity of bacteria and the crystal formation additive were optimized for the highest calcite yield, a maximum amount of 15.46 g calcite was precipitated from 100 mL of solution with a substrate concentration of 8%, which corresponds to a calcite yield of 28.7%.