TY - JOUR
T1 - Brain metabonomics study of ischemic cerebral apoplexy rats treated with Tongsaimai pellets
AU - Tu, Jia Yu
AU - A, Ji Ye
AU - Wen, Hong Mei
AU - Wang, Guang Ji
AU - Cao, Bei
AU - Liu, Lin Sheng
AU - Lu, Yi Hong
AU - Wang, Ai Yun
AU - Di, Liu Qing
PY - 2011/10
Y1 - 2011/10
N2 - Aim: To observe cerebral metabolic changes of ischemic cerebral apoplexy rat and the regulating action of Tongsaimai pellets on abnormal metabolism, and to find the potential biomarkers. To explore mechanisms of Tongsaimai pellets in treatment of ischemic cerebral apoplexy. Methods: Rat models of middle cerebral artery occlusion was established with electric coagulation, and rats were divided into 4 groups, model group, sham-operation group, Tongsaimai pellets group and positive control group. Endogenous metabolites of four groups were determined with GC-MS. Partial least squares discriminant analysis (PLS-DA) was applied to analyze multivariate data, and T-test was used in significant statistical analysis. Results: In scatter plot of rat brain samples, model group could be distinguished from 3 other groups. Compared with sham-operation group rats, alanine, serine, methio-nine, citric acid, histidine, arachidonic acid, docosahexaenoic acid increased in model group rats, while ribose, glucose-6-phosphate and inosine decreased, so these metabolites were potential metabolic biomarkers. These endogenous metabolites got restoration in Tongsaimai group rats. Arachidonic acid (AA) and docosahexaenoic acid (DHA) were statistically different between Tongsaimai pellets group and model group rats, showing that Tongsaimai pellets could obviously regulate AA and DHA. Conclusion: Changed metabolities can be certainly recovered by Tongsaimai pellets, and the treatment of Tongsaimai pellets can be connected with the regulation of related metabolic pathways.
AB - Aim: To observe cerebral metabolic changes of ischemic cerebral apoplexy rat and the regulating action of Tongsaimai pellets on abnormal metabolism, and to find the potential biomarkers. To explore mechanisms of Tongsaimai pellets in treatment of ischemic cerebral apoplexy. Methods: Rat models of middle cerebral artery occlusion was established with electric coagulation, and rats were divided into 4 groups, model group, sham-operation group, Tongsaimai pellets group and positive control group. Endogenous metabolites of four groups were determined with GC-MS. Partial least squares discriminant analysis (PLS-DA) was applied to analyze multivariate data, and T-test was used in significant statistical analysis. Results: In scatter plot of rat brain samples, model group could be distinguished from 3 other groups. Compared with sham-operation group rats, alanine, serine, methio-nine, citric acid, histidine, arachidonic acid, docosahexaenoic acid increased in model group rats, while ribose, glucose-6-phosphate and inosine decreased, so these metabolites were potential metabolic biomarkers. These endogenous metabolites got restoration in Tongsaimai group rats. Arachidonic acid (AA) and docosahexaenoic acid (DHA) were statistically different between Tongsaimai pellets group and model group rats, showing that Tongsaimai pellets could obviously regulate AA and DHA. Conclusion: Changed metabolities can be certainly recovered by Tongsaimai pellets, and the treatment of Tongsaimai pellets can be connected with the regulation of related metabolic pathways.
KW - GC-MS
KW - Ischemic cerebral apoplexy
KW - Metabonomics
KW - Potential biomarkers
KW - Rat brain
KW - Tongsaimai pellets
UR - https://www.scopus.com/pages/publications/84255176162
U2 - 10.3969/j.issn.1001-1978.2011.10.031
DO - 10.3969/j.issn.1001-1978.2011.10.031
M3 - 文章
AN - SCOPUS:84255176162
SN - 1001-1978
VL - 27
SP - 1467
EP - 1471
JO - Chinese Pharmacological Bulletin
JF - Chinese Pharmacological Bulletin
IS - 10
ER -