Biocompatibility Study of Crosslinked Hyaluronic Acid/β-tricalcium Phosphate Composite Scaffold Material

Xian Ya Xue; You Fa Wang; Rui Min Zhao

doi:10.3963/j.issn.1671-4431.2016.09.005

Biocompatibility Study of Crosslinked Hyaluronic Acid/β-tricalcium Phosphate Composite Scaffold Material

Xian Ya Xue
, You Fa Wang
, Rui Min Zhao

Wuhan University of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

The hyaluronic acid/β-tricalcium phosphate composite material was prepared by chemical crosslinking. The crosslinked and non-crosslinked HA/β-TCP composite scaffolds were co-cultured with bone marrow mesenchymal stem cells to evaluate the role of mitochondrial stress signaling pathway in the regulation of BMSCs apoptosis.We obtained the results of CCK-8 kit, cell adhesion assay, Hoechst/PI staining assay, flow cytometry for the detection of apoptotic rate, and Western Blot detection of Bax and Bcl-2-related proteins in mitochondrial mechanism. The experimental results show that the the crosslinked materials have higher viability and lower apoptosis than non-crosslinked scaffolds, this scaffold is expected to be widely used in cartilage tissue engineering.

Original language	English
Pages (from-to)	29-35
Number of pages	7
Journal	Wuhan Ligong Daxue Xuebao/Journal of Wuhan University of Technology
Volume	38
Issue number	9
DOIs	https://doi.org/10.3963/j.issn.1671-4431.2016.09.005
State	Published - 30 Sep 2016
Externally published	Yes

Keywords

Apoptosis
Bax
Bcl-2
BMSC
Mitochondrial
Scaffold

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10.3963/j.issn.1671-4431.2016.09.005

Cite this

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title = "Biocompatibility Study of Crosslinked Hyaluronic Acid/β-tricalcium Phosphate Composite Scaffold Material",

abstract = "The hyaluronic acid/β-tricalcium phosphate composite material was prepared by chemical crosslinking. The crosslinked and non-crosslinked HA/β-TCP composite scaffolds were co-cultured with bone marrow mesenchymal stem cells to evaluate the role of mitochondrial stress signaling pathway in the regulation of BMSCs apoptosis.We obtained the results of CCK-8 kit, cell adhesion assay, Hoechst/PI staining assay, flow cytometry for the detection of apoptotic rate, and Western Blot detection of Bax and Bcl-2-related proteins in mitochondrial mechanism. The experimental results show that the the crosslinked materials have higher viability and lower apoptosis than non-crosslinked scaffolds, this scaffold is expected to be widely used in cartilage tissue engineering.",

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T1 - Biocompatibility Study of Crosslinked Hyaluronic Acid/β-tricalcium Phosphate Composite Scaffold Material

AU - Xue, Xian Ya

AU - Wang, You Fa

AU - Zhao, Rui Min

PY - 2016/9/30

Y1 - 2016/9/30

N2 - The hyaluronic acid/β-tricalcium phosphate composite material was prepared by chemical crosslinking. The crosslinked and non-crosslinked HA/β-TCP composite scaffolds were co-cultured with bone marrow mesenchymal stem cells to evaluate the role of mitochondrial stress signaling pathway in the regulation of BMSCs apoptosis.We obtained the results of CCK-8 kit, cell adhesion assay, Hoechst/PI staining assay, flow cytometry for the detection of apoptotic rate, and Western Blot detection of Bax and Bcl-2-related proteins in mitochondrial mechanism. The experimental results show that the the crosslinked materials have higher viability and lower apoptosis than non-crosslinked scaffolds, this scaffold is expected to be widely used in cartilage tissue engineering.

AB - The hyaluronic acid/β-tricalcium phosphate composite material was prepared by chemical crosslinking. The crosslinked and non-crosslinked HA/β-TCP composite scaffolds were co-cultured with bone marrow mesenchymal stem cells to evaluate the role of mitochondrial stress signaling pathway in the regulation of BMSCs apoptosis.We obtained the results of CCK-8 kit, cell adhesion assay, Hoechst/PI staining assay, flow cytometry for the detection of apoptotic rate, and Western Blot detection of Bax and Bcl-2-related proteins in mitochondrial mechanism. The experimental results show that the the crosslinked materials have higher viability and lower apoptosis than non-crosslinked scaffolds, this scaffold is expected to be widely used in cartilage tissue engineering.

KW - Apoptosis

KW - Bax

KW - Bcl-2

KW - BMSC

KW - Mitochondrial

KW - Scaffold

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DO - 10.3963/j.issn.1671-4431.2016.09.005

M3 - 文章

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SN - 1671-4431

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JO - Wuhan Ligong Daxue Xuebao/Journal of Wuhan University of Technology

JF - Wuhan Ligong Daxue Xuebao/Journal of Wuhan University of Technology

IS - 9

ER -

Biocompatibility Study of Crosslinked Hyaluronic Acid/β-tricalcium Phosphate Composite Scaffold Material

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Keywords

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