Atomic force microscope investigation of large-circle DNA molecules

Aiguo Wu; Lihua Yu; Zhuang Li; Huanming Yang; Erkang Wang

doi:10.1016/j.ab.2003.11.005

Atomic force microscope investigation of large-circle DNA molecules

Aiguo Wu
, Lihua Yu
, Zhuang Li
, Huanming Yang
, Erkang Wang

CAS - Changchun Institute of Applied Chemistry
BGI-Shenzhen

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

A circular bacterial artificial chromosome of 148.9kbp on human chromosome 3 has been extended and fixed on bare mica substrates using a developed fluid capillary flow method in evaporating liquid drops. Extended circular DNA molecules were imaged with an atomic force microscope (AFM) under ambient conditions. The measured total lengths of the whole DNA molecules were in agreement with sequencing analysis data with an error range of ±3.6%. This work is important groundwork for probing single nucleotide polymorphisms in the human genome, mapping genomic DNA, manipulating biomolecular nanotechnology, and studying the interaction of DNA-protein complexes investigated by AFM.

Original language	English
Pages (from-to)	293-300
Number of pages	8
Journal	Analytical Biochemistry
Volume	325
Issue number	2
DOIs	https://doi.org/10.1016/j.ab.2003.11.005
State	Published - 15 Feb 2004
Externally published	Yes

Keywords

Atomic force microscopy/microscope
DNA
Developed fluid capillary flow method
Evaporating liquid drops
Human genome
Mica

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10.1016/j.ab.2003.11.005

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title = "Atomic force microscope investigation of large-circle DNA molecules",

abstract = "A circular bacterial artificial chromosome of 148.9kbp on human chromosome 3 has been extended and fixed on bare mica substrates using a developed fluid capillary flow method in evaporating liquid drops. Extended circular DNA molecules were imaged with an atomic force microscope (AFM) under ambient conditions. The measured total lengths of the whole DNA molecules were in agreement with sequencing analysis data with an error range of ±3.6\%. This work is important groundwork for probing single nucleotide polymorphisms in the human genome, mapping genomic DNA, manipulating biomolecular nanotechnology, and studying the interaction of DNA-protein complexes investigated by AFM.",

keywords = "Atomic force microscopy/microscope, DNA, Developed fluid capillary flow method, Evaporating liquid drops, Human genome, Mica",

author = "Aiguo Wu and Lihua Yu and Zhuang Li and Huanming Yang and Erkang Wang",

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doi = "10.1016/j.ab.2003.11.005",

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T1 - Atomic force microscope investigation of large-circle DNA molecules

AU - Wu, Aiguo

AU - Yu, Lihua

AU - Li, Zhuang

AU - Yang, Huanming

AU - Wang, Erkang

PY - 2004/2/15

Y1 - 2004/2/15

N2 - A circular bacterial artificial chromosome of 148.9kbp on human chromosome 3 has been extended and fixed on bare mica substrates using a developed fluid capillary flow method in evaporating liquid drops. Extended circular DNA molecules were imaged with an atomic force microscope (AFM) under ambient conditions. The measured total lengths of the whole DNA molecules were in agreement with sequencing analysis data with an error range of ±3.6%. This work is important groundwork for probing single nucleotide polymorphisms in the human genome, mapping genomic DNA, manipulating biomolecular nanotechnology, and studying the interaction of DNA-protein complexes investigated by AFM.

AB - A circular bacterial artificial chromosome of 148.9kbp on human chromosome 3 has been extended and fixed on bare mica substrates using a developed fluid capillary flow method in evaporating liquid drops. Extended circular DNA molecules were imaged with an atomic force microscope (AFM) under ambient conditions. The measured total lengths of the whole DNA molecules were in agreement with sequencing analysis data with an error range of ±3.6%. This work is important groundwork for probing single nucleotide polymorphisms in the human genome, mapping genomic DNA, manipulating biomolecular nanotechnology, and studying the interaction of DNA-protein complexes investigated by AFM.

KW - Atomic force microscopy/microscope

KW - DNA

KW - Developed fluid capillary flow method

KW - Evaporating liquid drops

KW - Human genome

KW - Mica

UR - https://www.scopus.com/pages/publications/1642578942

U2 - 10.1016/j.ab.2003.11.005

DO - 10.1016/j.ab.2003.11.005

M3 - 文章

C2 - 14751264

AN - SCOPUS:1642578942

SN - 0003-2697

VL - 325

SP - 293

EP - 300

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Atomic force microscope investigation of large-circle DNA molecules

Abstract

Keywords

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