Assay of malondialdehyde and other alkanals in biological fluids by gas chromatography-mass spectrometry

Helen C. Yeo; Jiankang Liu; Harold J. Helbock; Bruce N. Ames

doi:10.1016/S0076-6879(99)00115-9

Assay of malondialdehyde and other alkanals in biological fluids by gas chromatography-mass spectrometry

Helen C. Yeo
, Jiankang Liu
, Harold J. Helbock
, Bruce N. Ames

School of Life Science and Technology (SLST)

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

The method described in this chapter allows the accurate measurement of MDA in diverse biological samples and can be extended to measurements of other alkanals. The use of GC/MS-NCI ensures specificity and sensitivity, and the ability to prepare samples without heating limits oxidation artifact. Although the widely used TBA assay for MDA does not require sophisticated equipment, its results may be of limited value as the assay is hindered by the possibility of cross reactivity and by heat-induced oxidation artifact. This GC-MS technique offers the additional advantages of efficient processing of large numbers of samples and the elimination of recovery errors by inclusion of an internal standard.

Original language	English
Pages (from-to)	70-78
Number of pages	9
Journal	Methods in Enzymology
Volume	300
DOIs	https://doi.org/10.1016/S0076-6879(99)00115-9
State	Published - 1999

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title = "Assay of malondialdehyde and other alkanals in biological fluids by gas chromatography-mass spectrometry",

abstract = "The method described in this chapter allows the accurate measurement of MDA in diverse biological samples and can be extended to measurements of other alkanals. The use of GC/MS-NCI ensures specificity and sensitivity, and the ability to prepare samples without heating limits oxidation artifact. Although the widely used TBA assay for MDA does not require sophisticated equipment, its results may be of limited value as the assay is hindered by the possibility of cross reactivity and by heat-induced oxidation artifact. This GC-MS technique offers the additional advantages of efficient processing of large numbers of samples and the elimination of recovery errors by inclusion of an internal standard.",

author = "Yeo, \{Helen C.\} and Jiankang Liu and Helbock, \{Harold J.\} and Ames, \{Bruce N.\}",

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AU - Yeo, Helen C.

AU - Liu, Jiankang

AU - Helbock, Harold J.

AU - Ames, Bruce N.

PY - 1999

Y1 - 1999

N2 - The method described in this chapter allows the accurate measurement of MDA in diverse biological samples and can be extended to measurements of other alkanals. The use of GC/MS-NCI ensures specificity and sensitivity, and the ability to prepare samples without heating limits oxidation artifact. Although the widely used TBA assay for MDA does not require sophisticated equipment, its results may be of limited value as the assay is hindered by the possibility of cross reactivity and by heat-induced oxidation artifact. This GC-MS technique offers the additional advantages of efficient processing of large numbers of samples and the elimination of recovery errors by inclusion of an internal standard.

AB - The method described in this chapter allows the accurate measurement of MDA in diverse biological samples and can be extended to measurements of other alkanals. The use of GC/MS-NCI ensures specificity and sensitivity, and the ability to prepare samples without heating limits oxidation artifact. Although the widely used TBA assay for MDA does not require sophisticated equipment, its results may be of limited value as the assay is hindered by the possibility of cross reactivity and by heat-induced oxidation artifact. This GC-MS technique offers the additional advantages of efficient processing of large numbers of samples and the elimination of recovery errors by inclusion of an internal standard.

UR - https://www.scopus.com/pages/publications/0031770828

U2 - 10.1016/S0076-6879(99)00115-9

DO - 10.1016/S0076-6879(99)00115-9

M3 - 文章

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AN - SCOPUS:0031770828

SN - 0076-6879

VL - 300

SP - 70

EP - 78

JO - Methods in Enzymology

JF - Methods in Enzymology

ER -

Assay of malondialdehyde and other alkanals in biological fluids by gas chromatography-mass spectrometry

Abstract

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