Aqueous reactive species induced by a PCB surface micro-discharge air plasma device: a quantitative study

  • Chen Chen
  • , Fanying Li
  • , Hai Lan Chen
  • , Michael G. Kong

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

This paper presents a quantitative investigation on aqueous reactive species induced by air plasma generated from a printed circuit board surface micro-discharge (SMD) device. Under the conditions amenable for proliferation of mammalian cells, concentrations of ten types of reactive oxygen and nitrogen species (RONS) in phosphate buffering solution (PBS) are measured by chemical fluorescent assays and electron spin resonance spectroscopy (ESR). Results show that concentrations of several detected RNS (NO-2 , NO-3 , peroxynitrites, and NO-2) are higher than those of ROS (H2O2, O-2, and 1O2) in the air plasma treated solution. Concentrations of NO-3 can reach 150 times of H2O2 with 60 s plasma treatment. For shortlived species, the air plasma generates more copious peroxynitrite than other RONS including NO-2, O-2 , 1O2, and NO in PBS. In addition, the existence of reaction between H2O2 and NO-2/HNO2 to produce peroxynitrite is verified by the chemical scavenger experiments. The reaction relations between detected RONS are also discussed.

Original languageEnglish
Article number445208
JournalJournal of Physics D: Applied Physics
Volume50
Issue number44
DOIs
StatePublished - 12 Oct 2017

Keywords

  • air plasma
  • aqueous chemistry
  • peroxynitrite
  • reactive oxygen and nitrogen species
  • surface micro-discharge

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