TY - JOUR
T1 - Application of scanning ion conductance microscope in cell characterizations
AU - Lang, Jinxin
AU - Li, Yabei
AU - Yang, Yaowei
AU - Jiao, Yangbohan
AU - Wang, Meng
AU - Zhuang, Jian
AU - Li, Fei
N1 - Publisher Copyright:
© 2019 Scientia Sinica Chimica. All rights reserved.
PY - 2019
Y1 - 2019
N2 - Cell morphology, electrical and mechanical functions are of great significance in cell biology. However, traditional cell characterization methods are not suitable for study of living cells in a non-invasive, in situ and micro/ nano-scaled way. As a new type of scanning probe microscopy, scanning ion conductance microscopy (SICM) enables non-invasive, high-resolution and real-time imaging of living cells based on its non-contact scanning mode and usage of nanopipette as its probe. In recent years, SICM has been widely applied in cell biology and cell characterizations. This review mainly introduces the SICM applications in cell characterizations. First, we introduce the instrumental composition, working principle and three work modes of SICM, and compare the strengths and weakness of the three working modes. Then the applications of SICM in cell morphology, cellular electrical and mechanical properties are described in detail. In addition, in contrast to the traditional cell characterization methods, SICM has the distinct advantages in in situ characterization of cells. Finally, the challenges of SICM in cell characterizations are discussed and its future development is proposed.
AB - Cell morphology, electrical and mechanical functions are of great significance in cell biology. However, traditional cell characterization methods are not suitable for study of living cells in a non-invasive, in situ and micro/ nano-scaled way. As a new type of scanning probe microscopy, scanning ion conductance microscopy (SICM) enables non-invasive, high-resolution and real-time imaging of living cells based on its non-contact scanning mode and usage of nanopipette as its probe. In recent years, SICM has been widely applied in cell biology and cell characterizations. This review mainly introduces the SICM applications in cell characterizations. First, we introduce the instrumental composition, working principle and three work modes of SICM, and compare the strengths and weakness of the three working modes. Then the applications of SICM in cell morphology, cellular electrical and mechanical properties are described in detail. In addition, in contrast to the traditional cell characterization methods, SICM has the distinct advantages in in situ characterization of cells. Finally, the challenges of SICM in cell characterizations are discussed and its future development is proposed.
KW - Cell electrical properties
KW - Cell mechanical properties
KW - Cell morphology
KW - Scanning ion conductance microscope
UR - https://www.scopus.com/pages/publications/85081891001
U2 - 10.1360/N032018-00266
DO - 10.1360/N032018-00266
M3 - 文章
AN - SCOPUS:85081891001
SN - 1674-7224
VL - 49
SP - 844
EP - 860
JO - Scientia Sinica Chimica
JF - Scientia Sinica Chimica
IS - 6
ER -