Application of laser capture microdissection to capture pure cells in lung cancer and their paired normal lung tissues

Objective: Microheterogeneity is an important problem in molecularly biological and proteomic research on malignancies. The goal of this study was to investigate the method to purify targeted cells in lung cancer and its paired normal tissues for proteomic study on lung cancer applying laser capture microdissection (LCM). Methods: LCM technique was emploged to obtain the cells of lung cancer tissues and their paired normal tissues from frozen sections. Results: LCM can be applied to quickly and precisely obtain pure targeted cells subgroup or single cell without interstitium under the microscope. Conclusion: LCM can tackle the problem of tissue heterogeneity in molecular analysis successfully. These results indicate that LCM has a potential as a tool in lung cancer proteomic research.