Application of CD45/SSC gating in acute leukemia immunophenotyping

Objective: To investigate the application of CD45/SSC gating in acute leukemia immunophenotype. Methods: Three-color monoclonal antibodies were used to mark the marrows of acute leukemia patients, and CD45/SSC gating in flow cytometry was used for immunofluorescence. Results: The 49 cases of acute leukemia immunophenotype were classified into 3 subgroups, lineage specific antigen expression (25 cases), aberration antigen expression (21 cases), and null type (3 cases). The percentage of each subgroup was 51.2%, 42.8% and 6.0%, respectively. Conclusion: CD45/SSC gating technique can easily discriminate mature leukemia cells from normal cells, thus rendering more reliable and precise immunophenotype results.