Analysis on HLA haplotypes of loci HLA-A, -B, and -DRB1 in northwest Chinese Han population

Objective: To investigate HLA-A,-B and -DRB1 allele and HLA-A-B, B-DRB1, A-B-DRB1 haplotype frequencies in the northwest Chinese Han population. Methods: The authors investigated the HLA-A, -B, -DRB1 allele and haplotype in a northwest Chinese Han population based on 62 families and 101 individuals by use of PCR-sequence specific oligonucleotide probes (PCR-SSOP) DNA typing methods. Results: Fifteen alleles for the locus HLA-A, 28 alleles for the HLA-B locus and 13 alleles for the HLA-DRB1 were detected. The results showed that the most frequent HLA alleles found were A02 (0.3244), B13 (0.1200), and DRB1 * 15 (0.1400). Allele frequencies of more than 10% for HLA antigens were A02, A11, A24, B13, B15, B40, DRB1 * 04, DRB1 * 07, DRB1 * 09, DRB1 * 15. In the analysis of HLA haplotypes, 122 kinds of HLA-A-B haplotypes and 147 kinds HLA-B-DRB1 haplotypes were found. Two hundred and seventy-eight kinds of HLA-A-B-DRB1 haplotypes were found, comprising 61.78% (278/450) of total theoretical haplotypes. Eighty-three kinds of HLA-A-B-DRB1 haplotypes were shown to have at least two same haplotypes, comprising 18.44% (83/450) of total theoretical haplotypes. The most common HLA-A-B-DRB1 haplotypes were A30-B13-DRB1 * 07, A02-B46-DRB1 * 09, A01-B37-DRB1 * 10, A24-B15-DRB1 * 15, A02-B46-DRB1 * 08, A33-B58-DRB1 * 03. Conclusion: The data can be used for the estimation of the probability of finding haplotypically identical, related or unrelated bone marrow donor for an individual patient, and forensic and paternity tests to estimate the frequency of a DNA profile or anthropologic research.