Abstract
Objective: To screen potential specific biomarkers in the serum of lung tuberculosis patients using pre-fractionated magnetic beads based sample fractionation method combined with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) technology. Methods: We compared serum proteomic profiling of 14 lung tuberculosis patients and 32 healthy controls by MB-MALDI-TOF-MS (MB-WCX and MB-IMC-CU). Results: By analyzing the spectra (screened from the two groups) using the ClinProTools software 2.2, 42 and 11 peaks had significantly different expressions (P<0.001) between the two groups of all 74 and 68 peaks generated from analysis of MB-MALDI-TOF-MS (MB-WCX and MB-IMC-CU). In addition, 8 of the 11 peaks were the same as those in the 42 peaks, and 17 peaks were up-expressed while 25 were down-expressed in lung tuberculosis patients with 42 peaks. Conclusion: We were able to identify proteomic patterns that can clearly distinguish between patients with lung tuberculosis and healthy controls based on MB-MALDI-TOF-MS analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 728-732 |
| Number of pages | 5 |
| Journal | Journal of Xi'an Jiaotong University (Medical Sciences) |
| Volume | 32 |
| Issue number | 6 |
| State | Published - Nov 2011 |
Keywords
- Differentially expressed protein
- Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MB-MALDI-TOF-MS)
- Patient with lung tuberculosis
