An improved synthesis of water-soluble dual fluorescence emission carbon dots from holly leaves for accurate detection of mercury ions in living cells

Background: Carbon dots (CDs) emitting near-infrared fluorescence were recently synthesized from green leaves. However, the Hg²⁺ detection of CDs was limited because of the insufficient water solubility, low fluorescence and poor stability. Methods: Dual fluorescence emission water-soluble CD (Dual-CD) was prepared through a solvothermal method from holly leaves and low toxic PEI_1.8k. PEG was further grafted onto the surface to improve the water solubility and stability. Results: The Dual-CD solution can emit 487 nm and 676 nm fluorescence under single excitation and exhibit high quantum yield of 16.8%. The fluorescence at 678 nm decreased remarkably while the emission at 470 nm was slightly affected by the addition of Hg²⁺. The ratiometric Hg²⁺ detection had a wide linear range of 0–100 μM and low detection limit of 14.0 nM. In A549 cells, there was a good linear relation between F₄₈₇ /F₆₇₆ and the concentration of Hg²⁺ in the range of 0–60 μM; the detection limit was 477 nM. Furthermore, Dual-CD showed visual fluorescence change under Hg²⁺. Conclusion: Dual-CD has ratiometric responsiveness to Hg²⁺ and can be applied for quantitative Hg²⁺ detection in living cells.