Alteration of retinoid X receptor a mRNA level after acitretin and/or narrow-band UVB treatment in normal human keratinocytes

Su Ju Luo; Yan Zheng; Zhen Hui Peng; Guo Rong Wang; Shao Na Zhou; Xiao Li Li

Alteration of retinoid X receptor a mRNA level after acitretin and/or narrow-band UVB treatment in normal human keratinocytes

Su Ju Luo
, Yan Zheng
, Zhen Hui Peng
, Guo Rong Wang
, Shao Na Zhou
, Xiao Li Li

The Second Affiliated Hospital of Xi'an Jiaotong University

Research output: Contribution to journal › Article › peer-review

Abstract

Objective To investigate the alteration of retinoid X receptor α(RXRα) mRNA level in normal human keratinocytes after acitretin and/or NB-UVB irradiation treatment. Methods After a 12-hour incubation with 10^-7-10^-6 mol/L acitretin and/or following 50-100 mJ/cm² NB-UVB irradiation in normal human keratinocytes. RXRa mRNA expression was examined by reverse transcription polymerase chain reaction (RT-PCR) and real-time quantitative RT-PCR. Results The expression of RXRa mRNA was obviously decreased by NB-UVB irradiation, but not by acitretion single treatment. When combining acitretin treatment with NB-UVB irradiation, greater decreased RXRα mRNA expression was observed than that of single treatment. Conclusion Narrow-band UVB irradiation treatment can decrease RXRα mRNA expression, but not acitretin single treatment. Combining treatment with both can produce synergistic inhibition effects.

Original language	English
Pages (from-to)	455-457
Number of pages	3
Journal	Journal of Sichuan University (Medical Science Edition)
Volume	39
Issue number	3
State	Published - May 2008
Externally published	Yes

Keywords

Acitretin
Keratinocyte
Retinoic acid receptor
UVB

Cite this

@article{491167877aa847eca7d8d5b529c7840b,

title = "Alteration of retinoid X receptor a mRNA level after acitretin and/or narrow-band UVB treatment in normal human keratinocytes",

abstract = "Objective To investigate the alteration of retinoid X receptor α(RXRα) mRNA level in normal human keratinocytes after acitretin and/or NB-UVB irradiation treatment. Methods After a 12-hour incubation with 10-7-10-6 mol/L acitretin and/or following 50-100 mJ/cm2 NB-UVB irradiation in normal human keratinocytes. RXRa mRNA expression was examined by reverse transcription polymerase chain reaction (RT-PCR) and real-time quantitative RT-PCR. Results The expression of RXRa mRNA was obviously decreased by NB-UVB irradiation, but not by acitretion single treatment. When combining acitretin treatment with NB-UVB irradiation, greater decreased RXRα mRNA expression was observed than that of single treatment. Conclusion Narrow-band UVB irradiation treatment can decrease RXRα mRNA expression, but not acitretin single treatment. Combining treatment with both can produce synergistic inhibition effects.",

keywords = "Acitretin, Keratinocyte, Retinoic acid receptor, UVB",

author = "Luo, \{Su Ju\} and Yan Zheng and Peng, \{Zhen Hui\} and Wang, \{Guo Rong\} and Zhou, \{Shao Na\} and Li, \{Xiao Li\}",

year = "2008",

month = may,

language = "英语",

volume = "39",

pages = "455--457",

journal = "Journal of Sichuan University (Medical Science Edition)",

issn = "1672-173X",

publisher = "Sichuan University",

number = "3",

}

TY - JOUR

T1 - Alteration of retinoid X receptor a mRNA level after acitretin and/or narrow-band UVB treatment in normal human keratinocytes

AU - Luo, Su Ju

AU - Zheng, Yan

AU - Peng, Zhen Hui

AU - Wang, Guo Rong

AU - Zhou, Shao Na

AU - Li, Xiao Li

PY - 2008/5

Y1 - 2008/5

N2 - Objective To investigate the alteration of retinoid X receptor α(RXRα) mRNA level in normal human keratinocytes after acitretin and/or NB-UVB irradiation treatment. Methods After a 12-hour incubation with 10-7-10-6 mol/L acitretin and/or following 50-100 mJ/cm2 NB-UVB irradiation in normal human keratinocytes. RXRa mRNA expression was examined by reverse transcription polymerase chain reaction (RT-PCR) and real-time quantitative RT-PCR. Results The expression of RXRa mRNA was obviously decreased by NB-UVB irradiation, but not by acitretion single treatment. When combining acitretin treatment with NB-UVB irradiation, greater decreased RXRα mRNA expression was observed than that of single treatment. Conclusion Narrow-band UVB irradiation treatment can decrease RXRα mRNA expression, but not acitretin single treatment. Combining treatment with both can produce synergistic inhibition effects.

AB - Objective To investigate the alteration of retinoid X receptor α(RXRα) mRNA level in normal human keratinocytes after acitretin and/or NB-UVB irradiation treatment. Methods After a 12-hour incubation with 10-7-10-6 mol/L acitretin and/or following 50-100 mJ/cm2 NB-UVB irradiation in normal human keratinocytes. RXRa mRNA expression was examined by reverse transcription polymerase chain reaction (RT-PCR) and real-time quantitative RT-PCR. Results The expression of RXRa mRNA was obviously decreased by NB-UVB irradiation, but not by acitretion single treatment. When combining acitretin treatment with NB-UVB irradiation, greater decreased RXRα mRNA expression was observed than that of single treatment. Conclusion Narrow-band UVB irradiation treatment can decrease RXRα mRNA expression, but not acitretin single treatment. Combining treatment with both can produce synergistic inhibition effects.

KW - Acitretin

KW - Keratinocyte

KW - Retinoic acid receptor

KW - UVB

UR - https://www.scopus.com/pages/publications/44449127553

M3 - 文章

C2 - 18575340

AN - SCOPUS:44449127553

SN - 1672-173X

VL - 39

SP - 455

EP - 457

JO - Journal of Sichuan University (Medical Science Edition)

JF - Journal of Sichuan University (Medical Science Edition)

IS - 3

ER -

Alteration of retinoid X receptor a mRNA level after acitretin and/or narrow-band UVB treatment in normal human keratinocytes

Abstract

Keywords

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