Accurate and effective live bacteria microarray patterning on thick polycationic polymer layers co-patterned with HMDS

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Abstract

A new bacteria microarray patterning technique is developed by patterning thick polycationic polymers on a glass surface, which generates high-coverage and high-precision E. coli cell patterns. Cell immobilization efficiency is greatly improved, compared to that of the conventional monolayer surface patterning approach. Cell viability tests show very low cytotoxicity of polyethyleneimine (PEI). This advancement should further accelerate biomedical and bacteriological research on the micro scale.

Original languageEnglish
Pages (from-to)7673-7676
Number of pages4
JournalRSC Advances
Volume2
Issue number20
DOIs
StatePublished - 14 Sep 2012
Externally publishedYes

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