@article{3163e56b001346cf819dd2c15a5e0aee,
title = "A sensitive and specific genetically encoded potassium ion biosensor for in vivo applications across the tree of life",
abstract = "APUota:sPslieuamseicoonnf(iKrmthatallheadinglevelsarerepresentedcorrectly: +) plays a critical role as an essential electrolyte in all biological systems. Genetically encoded fluorescent K+ biosensors are promising tools to further improve our understanding of K+-dependent processes under normal and pathological conditions. Here, we report the crystal structure of a previously reported genetically encoded fluorescent K+ biosensor, GINKO1, in the K+-bound state. Using structure-guided optimization and directed evolution, we have engineered an improved K+ biosensor, designated GINKO2, with higher sensitivity and specificity. We have demonstrated the utility of GINKO2 for in vivo detection and imaging of K+ dynamics in multiple model organisms, including bacteria, plants, and mice.",
author = "Wu, \{Sheng Yi\} and Yurong Wen and Serre, \{Nelson B.C.\} and Laursen, \{Cathrine Charlotte Heiede\} and Dietz, \{Andrea Grost{\o}l\} and Taylor, \{Brian R.\} and Mikhail Drobizhev and Molina, \{Rosana S.\} and Abhi Aggarwal and Vladimir Rancic and Michael Becker and Klaus Ballanyi and Kaspar Podgorski and Hajime Hirase and Maiken Nedergaard and Maty{\'a}{\v s} Fendrych and Lemieux, \{M. Joanne\} and Eberl, \{Daniel F.\} and Kay, \{Alan R.\} and Campbell, \{Robert E.\} and Yi Shen",
note = "Publisher Copyright: {\textcopyright} 2022 This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.",
year = "2022",
month = sep,
doi = "10.1371/journal.pbio.3001772",
language = "英语",
volume = "20",
journal = "PLoS Biology",
issn = "1544-9173",
publisher = "Public Library of Science",
number = "9",
}