TY - GEN
T1 - A QCM biosensor based on gold nanoparticles amplification for real-time bacteria DNA detection
AU - Wang, Lijiang
AU - Wei, Qingshan
AU - Wu, Chunsheng
AU - Ji, Jian
AU - Wang, Ping
PY - 2007
Y1 - 2007
N2 - A DNA piezoelectric biosensing method for real-time detection of E.coli O157:H7 in a circulating-flow system was developed in this study. The thioled surface of the Au electrode could be immobilized by many inner Au nanoparticles, then more thiolated single-stranded DNA (ssDNA) probes which was specific to E. coli O157:H7 eaeA gene could be fixed through Au-SH bonding. The hybridization was induced by exposing the ssDNA probe to the complementary target DNA, then resulted in the mass change and corresponding frequency shifts (Δf) of the QCM. The outer avidin-coated Au nanoparticles could combine with the target DNA to increase the mass. The inner and outer Au nanoparticles with different diameters would play the "signal amplifier" role at different layer for improving the detection limit and sensitivity. The electrochemical techniques, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), are adopted to demonstrate and character each treatment. The target DNA corresponding to 2.0 × 103 colony forming unit (CFU)/ml E. coli O157:H7 cells can be detected by this biosensor, so it is practical to develop a sensitive and effective QCM biosensor for pathogenic bacteria detection based on specific DNA analysis.
AB - A DNA piezoelectric biosensing method for real-time detection of E.coli O157:H7 in a circulating-flow system was developed in this study. The thioled surface of the Au electrode could be immobilized by many inner Au nanoparticles, then more thiolated single-stranded DNA (ssDNA) probes which was specific to E. coli O157:H7 eaeA gene could be fixed through Au-SH bonding. The hybridization was induced by exposing the ssDNA probe to the complementary target DNA, then resulted in the mass change and corresponding frequency shifts (Δf) of the QCM. The outer avidin-coated Au nanoparticles could combine with the target DNA to increase the mass. The inner and outer Au nanoparticles with different diameters would play the "signal amplifier" role at different layer for improving the detection limit and sensitivity. The electrochemical techniques, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), are adopted to demonstrate and character each treatment. The target DNA corresponding to 2.0 × 103 colony forming unit (CFU)/ml E. coli O157:H7 cells can be detected by this biosensor, so it is practical to develop a sensitive and effective QCM biosensor for pathogenic bacteria detection based on specific DNA analysis.
UR - https://www.scopus.com/pages/publications/47349090524
U2 - 10.1109/ICIA.2007.4295695
DO - 10.1109/ICIA.2007.4295695
M3 - 会议稿件
AN - SCOPUS:47349090524
SN - 1424412196
SN - 9781424412198
T3 - Proceedings of the 2007 International Conference on Information Acquisition, ICIA
SP - 46
EP - 51
BT - 2007 International Conference on Information Acquisition, ICIA
T2 - International Conference on Information Acquisition, ICIA 2007
Y2 - 9 July 2007 through 11 July 2007
ER -