A novel substrate for in situ synthesis of oligonucleotides: Hydrolyzed microporous polyamide-6 membrane

Song Li; Jian Xin Tang; Mei Ju Ji; Peng Hou; Peng Feng Xiao; Nong Yue He

A novel substrate for in situ synthesis of oligonucleotides: Hydrolyzed microporous polyamide-6 membrane

Song Li
, Jian Xin Tang
, Mei Ju Ji
, Peng Hou
, Peng Feng Xiao
, Nong Yue He

Southeast University, Nanjing

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A novel substrate for in situ synthesis of oligonucleotide was prepared by hydrolyzing microporous polyamide-6 membranes in a 0.01 mol/L NaOH/(H ₂O-CH₃OH) mixture medium. The formation of amines (NH ₂) on the surface was proved by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The treated membrane was applied for in situ synthesis of oligonucleotide and a single step coupling efficiency determined by ultraviolet (UV) spectra was above 98 %.

Original language	English
Pages (from-to)	837-840
Number of pages	4
Journal	Chinese Chemical Letters
Volume	15
Issue number	7
State	Published - Jul 2004
Externally published	Yes

Keywords

Catalyzed hydrolysis
Coupling efficiency
Polyamide-6 membrane
in situ synthesis of oligonucleotides

Cite this

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title = "A novel substrate for in situ synthesis of oligonucleotides: Hydrolyzed microporous polyamide-6 membrane",

abstract = "A novel substrate for in situ synthesis of oligonucleotide was prepared by hydrolyzing microporous polyamide-6 membranes in a 0.01 mol/L NaOH/(H 2O-CH3OH) mixture medium. The formation of amines (NH 2) on the surface was proved by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The treated membrane was applied for in situ synthesis of oligonucleotide and a single step coupling efficiency determined by ultraviolet (UV) spectra was above 98 \%.",

keywords = "Catalyzed hydrolysis, Coupling efficiency, Polyamide-6 membrane, in situ synthesis of oligonucleotides",

author = "Song Li and Tang, \{Jian Xin\} and Ji, \{Mei Ju\} and Peng Hou and Xiao, \{Peng Feng\} and He, \{Nong Yue\}",

year = "2004",

month = jul,

language = "英语",

volume = "15",

pages = "837--840",

journal = "Chinese Chemical Letters",

issn = "1001-8417",

publisher = "Elsevier B.V.",

number = "7",

}

TY - JOUR

T1 - A novel substrate for in situ synthesis of oligonucleotides

T2 - Hydrolyzed microporous polyamide-6 membrane

AU - Li, Song

AU - Tang, Jian Xin

AU - Ji, Mei Ju

AU - Hou, Peng

AU - Xiao, Peng Feng

AU - He, Nong Yue

PY - 2004/7

Y1 - 2004/7

N2 - A novel substrate for in situ synthesis of oligonucleotide was prepared by hydrolyzing microporous polyamide-6 membranes in a 0.01 mol/L NaOH/(H 2O-CH3OH) mixture medium. The formation of amines (NH 2) on the surface was proved by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The treated membrane was applied for in situ synthesis of oligonucleotide and a single step coupling efficiency determined by ultraviolet (UV) spectra was above 98 %.

AB - A novel substrate for in situ synthesis of oligonucleotide was prepared by hydrolyzing microporous polyamide-6 membranes in a 0.01 mol/L NaOH/(H 2O-CH3OH) mixture medium. The formation of amines (NH 2) on the surface was proved by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The treated membrane was applied for in situ synthesis of oligonucleotide and a single step coupling efficiency determined by ultraviolet (UV) spectra was above 98 %.

KW - Catalyzed hydrolysis

KW - Coupling efficiency

KW - Polyamide-6 membrane

KW - in situ synthesis of oligonucleotides

UR - https://www.scopus.com/pages/publications/4444348693

M3 - 文章

AN - SCOPUS:4444348693

SN - 1001-8417

VL - 15

SP - 837

EP - 840

JO - Chinese Chemical Letters

JF - Chinese Chemical Letters

IS - 7

ER -

A novel substrate for in situ synthesis of oligonucleotides: Hydrolyzed microporous polyamide-6 membrane

Abstract

Keywords

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