TY - JOUR
T1 - A novel role for DYX1C1, a chaperone protein for both Hsp70 and Hsp90, in breast cancer
AU - Chen, Yuxin
AU - Zhao, Muzi
AU - Wang, Saiqun
AU - Chen, Jie
AU - Wang, Yun
AU - Cao, Qinhong
AU - Zhou, Wenbin
AU - Liu, Jin
AU - Xu, Zhiyang
AU - Tong, Guoqing
AU - Li, Jianmin
PY - 2009/9
Y1 - 2009/9
N2 - With three consecutive tetratricopeptide repeat (TPR) motifs at its C-terminus essential for neuronal migration, and a p23 domain at its N-terminus, DYX1C1 was the first gene proposed to have a role in developmental dyslexia. In this study, we attempted to identify the potential interaction of DYX1C1 and heat shock protein, and the role of DYX1C1 in breast cancer. GST pull-down, a yeast two-hybrid system, RT-PCR, site-directed mutagenesis approach. Our study initially confirmed DYX1C1, a dyslexia related protein, could interact with Hsp70 and Hsp90 via GST pull-down and a yeast two-hybrid system. And we verified that EEVD, the C-terminal residues of DYX1C1, is responsible for the identified association. Further, DYX1C1 mRNA was significantly overexpressed in malignant breast tumor, linking with the up-regulated expression of Hsp70 and Hsp90. These results suggest that DYX1C1 is a novel Hsp70 and Hsp90-interacting co-chaperone protein and its expression is associated with malignancy.
AB - With three consecutive tetratricopeptide repeat (TPR) motifs at its C-terminus essential for neuronal migration, and a p23 domain at its N-terminus, DYX1C1 was the first gene proposed to have a role in developmental dyslexia. In this study, we attempted to identify the potential interaction of DYX1C1 and heat shock protein, and the role of DYX1C1 in breast cancer. GST pull-down, a yeast two-hybrid system, RT-PCR, site-directed mutagenesis approach. Our study initially confirmed DYX1C1, a dyslexia related protein, could interact with Hsp70 and Hsp90 via GST pull-down and a yeast two-hybrid system. And we verified that EEVD, the C-terminal residues of DYX1C1, is responsible for the identified association. Further, DYX1C1 mRNA was significantly overexpressed in malignant breast tumor, linking with the up-regulated expression of Hsp70 and Hsp90. These results suggest that DYX1C1 is a novel Hsp70 and Hsp90-interacting co-chaperone protein and its expression is associated with malignancy.
KW - Breast cancer
KW - DYX1C1
KW - Heat shock protein
KW - Protein interaction
KW - Tetratricopeptide repeats
UR - https://www.scopus.com/pages/publications/68649089236
U2 - 10.1007/s00432-009-0568-6
DO - 10.1007/s00432-009-0568-6
M3 - 文章
C2 - 19277710
AN - SCOPUS:68649089236
SN - 0171-5216
VL - 135
SP - 1265
EP - 1276
JO - Journal of Cancer Research and Clinical Oncology
JF - Journal of Cancer Research and Clinical Oncology
IS - 9
ER -
