A novel phosphonic acid functional polythiophene fluorescent sensor for Ca2+ and its live cell imaging

Ting Yang; Zhiru Hu; Jilin Liu; Zhiquan Zhang; Guodong Feng

doi:10.1039/c9ay01318k

A novel phosphonic acid functional polythiophene fluorescent sensor for Ca²⁺ and its live cell imaging

Ting Yang
, Zhiru Hu
, Jilin Liu
, Zhiquan Zhang
, Guodong Feng

Jilin University

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Calcium ions are indispensable to various physiological activities of the body, so the detection of calcium ions is very important. In this work, a new water-soluble polythiophene derivative poly{((thiophene-3,4-diylbis(oxy))bis(propane-3,1-diyl))bis(phosphonic acid)} (PT-PHO) was designed and synthesized via oxidative polymerization with FeCl₃. The polymer containing phosphonic acid groups could be used as a "turn off" fluorescent sensor for Ca²⁺ detection. Under optimal conditions, PT-PHO exhibits high selectivity and sensitivity to detect Ca²⁺ among metal ions. The fluorescence intensity of PT-PHO shows a linear response toward Ca²⁺ in the range of 0.05-50 μmol L^-1 with a limit of detection (LOD) of 44 nmol L^-1. In addition, PT-PHO has low toxicity and good biocompatibility. Thus, it could be used for imaging lysosomes in cells and detecting intracellular calcium ions.

Original language	English
Pages (from-to)	4991-4997
Number of pages	7
Journal	Analytical Methods
Volume	11
Issue number	39
DOIs	https://doi.org/10.1039/c9ay01318k
State	Published - 21 Oct 2019
Externally published	Yes

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title = "A novel phosphonic acid functional polythiophene fluorescent sensor for Ca2+ and its live cell imaging",

abstract = "Calcium ions are indispensable to various physiological activities of the body, so the detection of calcium ions is very important. In this work, a new water-soluble polythiophene derivative poly\{((thiophene-3,4-diylbis(oxy))bis(propane-3,1-diyl))bis(phosphonic acid)\} (PT-PHO) was designed and synthesized via oxidative polymerization with FeCl3. The polymer containing phosphonic acid groups could be used as a {"}turn off{"} fluorescent sensor for Ca2+ detection. Under optimal conditions, PT-PHO exhibits high selectivity and sensitivity to detect Ca2+ among metal ions. The fluorescence intensity of PT-PHO shows a linear response toward Ca2+ in the range of 0.05-50 μmol L-1 with a limit of detection (LOD) of 44 nmol L-1. In addition, PT-PHO has low toxicity and good biocompatibility. Thus, it could be used for imaging lysosomes in cells and detecting intracellular calcium ions.",

author = "Ting Yang and Zhiru Hu and Jilin Liu and Zhiquan Zhang and Guodong Feng",

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year = "2019",

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doi = "10.1039/c9ay01318k",

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T1 - A novel phosphonic acid functional polythiophene fluorescent sensor for Ca2+ and its live cell imaging

AU - Yang, Ting

AU - Hu, Zhiru

AU - Liu, Jilin

AU - Zhang, Zhiquan

AU - Feng, Guodong

PY - 2019/10/21

Y1 - 2019/10/21

N2 - Calcium ions are indispensable to various physiological activities of the body, so the detection of calcium ions is very important. In this work, a new water-soluble polythiophene derivative poly{((thiophene-3,4-diylbis(oxy))bis(propane-3,1-diyl))bis(phosphonic acid)} (PT-PHO) was designed and synthesized via oxidative polymerization with FeCl3. The polymer containing phosphonic acid groups could be used as a "turn off" fluorescent sensor for Ca2+ detection. Under optimal conditions, PT-PHO exhibits high selectivity and sensitivity to detect Ca2+ among metal ions. The fluorescence intensity of PT-PHO shows a linear response toward Ca2+ in the range of 0.05-50 μmol L-1 with a limit of detection (LOD) of 44 nmol L-1. In addition, PT-PHO has low toxicity and good biocompatibility. Thus, it could be used for imaging lysosomes in cells and detecting intracellular calcium ions.

AB - Calcium ions are indispensable to various physiological activities of the body, so the detection of calcium ions is very important. In this work, a new water-soluble polythiophene derivative poly{((thiophene-3,4-diylbis(oxy))bis(propane-3,1-diyl))bis(phosphonic acid)} (PT-PHO) was designed and synthesized via oxidative polymerization with FeCl3. The polymer containing phosphonic acid groups could be used as a "turn off" fluorescent sensor for Ca2+ detection. Under optimal conditions, PT-PHO exhibits high selectivity and sensitivity to detect Ca2+ among metal ions. The fluorescence intensity of PT-PHO shows a linear response toward Ca2+ in the range of 0.05-50 μmol L-1 with a limit of detection (LOD) of 44 nmol L-1. In addition, PT-PHO has low toxicity and good biocompatibility. Thus, it could be used for imaging lysosomes in cells and detecting intracellular calcium ions.

UR - https://www.scopus.com/pages/publications/85073595457

U2 - 10.1039/c9ay01318k

DO - 10.1039/c9ay01318k

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SN - 1759-9660

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SP - 4991

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JO - Analytical Methods

JF - Analytical Methods

IS - 39

ER -

A novel phosphonic acid functional polythiophene fluorescent sensor for Ca²⁺ and its live cell imaging

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