A mitochondrial targeted two-photon iridium(III) phosphorescent probe for selective detection of hypochlorite in live cells and invivo

Endogenous hypochlorite ion (ClO^-) is a highly reactive oxygen species (ROS) that is produced from hydrogen peroxide and chloride ions catalyzed by myeloperoxidase (MPO). And mitochondrion is one of the major sources of ROS including ClO^-. In the present work, a two-photon phosphorescent probe for ClO^- in mitochondria was developed. An iridium(III) complex bearing a diaminomaleonitrile group as ClO^- reactive moiety specifically responded to ClO^- over other ions and ROSs. When the probe was reacted with ClO^- to form an oxidized carboxylate product, a significant enhancement in phosphorescence intensity was observed under one-photon (402nm) and two-photon (750nm) excitation, with a two-photon absorption cross-section of 78.1GM at 750nm. More importantly, ICP-MS results and cellular images co-stained with Mito-tracker Green demonstrated that this probe possessed high specificity for mitochondria. This probe was applied in the one- and two-photon imaging of ClO^- invitro and invivo. The results suggested endotoxin lipopolysaccharide (LPS) induced ClO^- mostly generated in the liver of zebrafish.