A highly-efficient imprinted magnetic nanoparticle for selective separation and detection of 17β-estradiol in milk

In this work, we prepared molecularly imprinted polymers (MIPs) combining surface molecular imprinting technique and magnetic separation for separation and determination of 17β-estradiol (E2) from milk. During the synthesis process, the acryloyl chloride was specially used to graft double bonds on Fe₃O₄ nanoparticles and served as co-functional monomer cooperating with acrylamide. The morphology, structure property, and the best polymerization and adsorption conditions of the prepared magnetic nanoparticles were investigated in detail. The obtained nanomaterials displayed high adsorption capacity of 12.62 mg/g, fast equilibrium time of 10 min, and satisfactory selectivity for target molecule. What's more, the MIPs was successfully applied as sorbents to specifically separate and enrich E2 from milk with a relatively high recovery (88.9-92.1%), demonstrating the potential application of the MIPs as solid phase extractant for rapid, highly-efficient, and cost-effective sample analysis.