A Fast Dual-responsive OFF-ON Fluorescent Probe for Cysteine and Glutathione without Interference from Homocysteine

Qingjie Fu; Ruxin Liu; Xiaoqian Cui; Yuqi Zhao; Hui Chi; Yongzhuang Lu; Qiang Fei; Guodong Feng; Hongyan Shan; Yanfu Huan

doi:10.2116/analsci.21P088

A Fast Dual-responsive OFF-ON Fluorescent Probe for Cysteine and Glutathione without Interference from Homocysteine

Qingjie Fu
, Ruxin Liu
, Xiaoqian Cui
, Yuqi Zhao
, Hui Chi
, Yongzhuang Lu
, Qiang Fei
, Guodong Feng
, Hongyan Shan
, Yanfu Huan

Jilin University

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Abnormal levels of biothiols, such as cysteine (Cys), homocystine (Hcy), and glutathione (GSH), are generally known to result in various diseases. A fast dual-responsive OFF-ON fluorescent probe HBO-AC was synthesized and developed. Non-fluorescent HBO-AC can sense Cys by regaining fluorescence at 444 nm within 10 min and a response to GSH by restoring fluorescence at 349 nm within 20 min. There is no mutual interference with Δλ ca. 100 nm. A novel method was developed by utilizing a low reaction rate between HBO-AC and Hcy to eliminate common interference from Hcy. A successful determination of Cys and GSH in fetal bovine serum (FBS) indicated that the probe had potential application for clinical diagnosis.

Original language	English
Pages (from-to)	1541-1546
Number of pages	6
Journal	Analytical Sciences
Volume	37
Issue number	11
DOIs	https://doi.org/10.2116/analsci.21P088
State	Published - 2021
Externally published	Yes

Keywords

Organic small molecule
biothiols discrimination
dual responsive
fast sensing
fluorescent off-on probe
interference elimination

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10.2116/analsci.21P088

Cite this

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title = "A Fast Dual-responsive OFF-ON Fluorescent Probe for Cysteine and Glutathione without Interference from Homocysteine",

abstract = "Abnormal levels of biothiols, such as cysteine (Cys), homocystine (Hcy), and glutathione (GSH), are generally known to result in various diseases. A fast dual-responsive OFF-ON fluorescent probe HBO-AC was synthesized and developed. Non-fluorescent HBO-AC can sense Cys by regaining fluorescence at 444 nm within 10 min and a response to GSH by restoring fluorescence at 349 nm within 20 min. There is no mutual interference with Δλ ca. 100 nm. A novel method was developed by utilizing a low reaction rate between HBO-AC and Hcy to eliminate common interference from Hcy. A successful determination of Cys and GSH in fetal bovine serum (FBS) indicated that the probe had potential application for clinical diagnosis.",

keywords = "Organic small molecule, biothiols discrimination, dual responsive, fast sensing, fluorescent off-on probe, interference elimination",

author = "Qingjie Fu and Ruxin Liu and Xiaoqian Cui and Yuqi Zhao and Hui Chi and Yongzhuang Lu and Qiang Fei and Guodong Feng and Hongyan Shan and Yanfu Huan",

note = "Publisher Copyright: {\textcopyright} The Japan Society for Analytical Chemistry",

year = "2021",

doi = "10.2116/analsci.21P088",

language = "英语",

volume = "37",

pages = "1541--1546",

journal = "Analytical Sciences",

issn = "0910-6340",

publisher = "Springer International Publishing AG",

number = "11",

}

TY - JOUR

T1 - A Fast Dual-responsive OFF-ON Fluorescent Probe for Cysteine and Glutathione without Interference from Homocysteine

AU - Fu, Qingjie

AU - Liu, Ruxin

AU - Cui, Xiaoqian

AU - Zhao, Yuqi

AU - Chi, Hui

AU - Lu, Yongzhuang

AU - Fei, Qiang

AU - Feng, Guodong

AU - Shan, Hongyan

AU - Huan, Yanfu

N1 - Publisher Copyright: © The Japan Society for Analytical Chemistry

PY - 2021

Y1 - 2021

N2 - Abnormal levels of biothiols, such as cysteine (Cys), homocystine (Hcy), and glutathione (GSH), are generally known to result in various diseases. A fast dual-responsive OFF-ON fluorescent probe HBO-AC was synthesized and developed. Non-fluorescent HBO-AC can sense Cys by regaining fluorescence at 444 nm within 10 min and a response to GSH by restoring fluorescence at 349 nm within 20 min. There is no mutual interference with Δλ ca. 100 nm. A novel method was developed by utilizing a low reaction rate between HBO-AC and Hcy to eliminate common interference from Hcy. A successful determination of Cys and GSH in fetal bovine serum (FBS) indicated that the probe had potential application for clinical diagnosis.

AB - Abnormal levels of biothiols, such as cysteine (Cys), homocystine (Hcy), and glutathione (GSH), are generally known to result in various diseases. A fast dual-responsive OFF-ON fluorescent probe HBO-AC was synthesized and developed. Non-fluorescent HBO-AC can sense Cys by regaining fluorescence at 444 nm within 10 min and a response to GSH by restoring fluorescence at 349 nm within 20 min. There is no mutual interference with Δλ ca. 100 nm. A novel method was developed by utilizing a low reaction rate between HBO-AC and Hcy to eliminate common interference from Hcy. A successful determination of Cys and GSH in fetal bovine serum (FBS) indicated that the probe had potential application for clinical diagnosis.

KW - Organic small molecule

KW - biothiols discrimination

KW - dual responsive

KW - fast sensing

KW - fluorescent off-on probe

KW - interference elimination

UR - https://www.scopus.com/pages/publications/85119651937

U2 - 10.2116/analsci.21P088

DO - 10.2116/analsci.21P088

M3 - 文章

C2 - 33896881

AN - SCOPUS:85119651937

SN - 0910-6340

VL - 37

SP - 1541

EP - 1546

JO - Analytical Sciences

JF - Analytical Sciences

IS - 11

ER -

A Fast Dual-responsive OFF-ON Fluorescent Probe for Cysteine and Glutathione without Interference from Homocysteine

Abstract

Keywords

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