Abstract
Objective: To investigate the effect of lentinan on Sorafenib resistance in hepatocellular carcinoma (HCC) cells BEL-7402/S and its mechanism. Methods: CCK-8 method was used to detect the proliferation-toxicity of lentinan and Sorafenib on BEL-7402 and BEL-7402/S cells, and the drug resistance reversal multiple of Sorafenib. The effects of lentinan on mRNA and protein expressions of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor (HIF-1α) in BEL-7402/S cells were determined by RT-qPCR and Western blotting. Results: Lentinan reduced the proliferation of BEL-7402 and BEL-7402/S cells. Lentinan showed at least a partial reversal of drug resistance to sorafenib in BEL-7402/S cells and down-regulated the mRNA and protein expressions of VEGF and HIF-1α in BEL-7402/S cells. Conclusion: Lentinan can partially reverse the effect of BEL-7402/S cells on Sorafenib resistance, and the mechanism may be related to the down-regulation of VEGF and HIF-1α expressions.
| Translated title of the contribution | Reverse effect of lentinanin on Sorafenib resistance in cultured hepatocellular carcinoma cells and its mechanism |
|---|---|
| Original language | Chinese (Traditional) |
| Pages (from-to) | 48-52 and 64 |
| Journal | Journal of Xi'an Jiaotong University (Medical Sciences) |
| Volume | 42 |
| Issue number | 1 |
| DOIs | |
| State | Published - 5 Jan 2021 |
| Externally published | Yes |
Fingerprint
Dive into the research topics of 'Reverse effect of lentinanin on Sorafenib resistance in cultured hepatocellular carcinoma cells and its mechanism'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver