转基因小鼠源性胰腺癌组织块皮下移植瘤模型的构建与评价

Wei Kun Qian; Jie Li; Bin Yan; Jun Yu Cao; Ying Xiao; Tao Qin; Qing Yong Ma; Liang Han

doi:10.7652/jdyxb201903006

转基因小鼠源性胰腺癌组织块皮下移植瘤模型的构建与评价

Translated title of the contribution: Establishment of transgenic mice-derived allografts in pancreatic cancer

Wei Kun Qian
, Jie Li
, Bin Yan
, Jun Yu Cao
, Ying Xiao
, Tao Qin
, Qing Yong Ma
, Liang Han

The First Affiliated Hospital of Xi’an Jiaotong University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Objective: To establish the transgenic mice-derived allografts (MDAs) so as to provide an appropriate experiment model of pancreatic cancer. Methods: By using the tissue fragments derived from transgenic mice with pancreatic cancer named LSL-Kras^G12D/+; Trp53^fl/+; Pdx1-Cre (KPC) mice and subcutaneous transplant technique, we constructed the MDAs. We evaluated the histopathological characteristics of MDAs and infiltration of immune cells by H&E staining, Masson's staining and immunohistochemical method. Results: MDAs could mimic the pathological morphology, proliferation and fibrosis of pancreatic cancer precisely. Besides, the infiltration of immune cells in MDAs tumor was the same as that of pancreatic cancer. Conclusion: MDAs are an effective model to mimic the development of pancreatic cancer.

Translated title of the contribution	Establishment of transgenic mice-derived allografts in pancreatic cancer
Original language	Chinese (Traditional)
Pages (from-to)	368-373
Number of pages	6
Journal	Journal of Xi'an Jiaotong University (Medical Sciences)
Volume	40
Issue number	3
DOIs	https://doi.org/10.7652/jdyxb201903006
State	Published - May 2019
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.7652/jdyxb201903006

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title = "转基因小鼠源性胰腺癌组织块皮下移植瘤模型的构建与评价",

abstract = "Objective: To establish the transgenic mice-derived allografts (MDAs) so as to provide an appropriate experiment model of pancreatic cancer. Methods: By using the tissue fragments derived from transgenic mice with pancreatic cancer named LSL-KrasG12D/+; Trp53fl/+; Pdx1-Cre (KPC) mice and subcutaneous transplant technique, we constructed the MDAs. We evaluated the histopathological characteristics of MDAs and infiltration of immune cells by H\&E staining, Masson's staining and immunohistochemical method. Results: MDAs could mimic the pathological morphology, proliferation and fibrosis of pancreatic cancer precisely. Besides, the infiltration of immune cells in MDAs tumor was the same as that of pancreatic cancer. Conclusion: MDAs are an effective model to mimic the development of pancreatic cancer.",

keywords = "Pancreatic cancer, Subcutaneous transplanted tumor, Tissue fragment, Transgenic mouse",

author = "Qian, \{Wei Kun\} and Jie Li and Bin Yan and Cao, \{Jun Yu\} and Ying Xiao and Tao Qin and Ma, \{Qing Yong\} and Liang Han",

year = "2019",

month = may,

doi = "10.7652/jdyxb201903006",

language = "繁体中文",

volume = "40",

pages = "368--373",

journal = "Journal of Xi'an Jiaotong University (Medical Sciences)",

issn = "1671-8259",

publisher = "Xi'an Medical University",

number = "3",

}

TY - JOUR

T1 - 转基因小鼠源性胰腺癌组织块皮下移植瘤模型的构建与评价

AU - Qian, Wei Kun

AU - Li, Jie

AU - Yan, Bin

AU - Cao, Jun Yu

AU - Xiao, Ying

AU - Qin, Tao

AU - Ma, Qing Yong

AU - Han, Liang

PY - 2019/5

Y1 - 2019/5

N2 - Objective: To establish the transgenic mice-derived allografts (MDAs) so as to provide an appropriate experiment model of pancreatic cancer. Methods: By using the tissue fragments derived from transgenic mice with pancreatic cancer named LSL-KrasG12D/+; Trp53fl/+; Pdx1-Cre (KPC) mice and subcutaneous transplant technique, we constructed the MDAs. We evaluated the histopathological characteristics of MDAs and infiltration of immune cells by H&E staining, Masson's staining and immunohistochemical method. Results: MDAs could mimic the pathological morphology, proliferation and fibrosis of pancreatic cancer precisely. Besides, the infiltration of immune cells in MDAs tumor was the same as that of pancreatic cancer. Conclusion: MDAs are an effective model to mimic the development of pancreatic cancer.

AB - Objective: To establish the transgenic mice-derived allografts (MDAs) so as to provide an appropriate experiment model of pancreatic cancer. Methods: By using the tissue fragments derived from transgenic mice with pancreatic cancer named LSL-KrasG12D/+; Trp53fl/+; Pdx1-Cre (KPC) mice and subcutaneous transplant technique, we constructed the MDAs. We evaluated the histopathological characteristics of MDAs and infiltration of immune cells by H&E staining, Masson's staining and immunohistochemical method. Results: MDAs could mimic the pathological morphology, proliferation and fibrosis of pancreatic cancer precisely. Besides, the infiltration of immune cells in MDAs tumor was the same as that of pancreatic cancer. Conclusion: MDAs are an effective model to mimic the development of pancreatic cancer.

KW - Pancreatic cancer

KW - Subcutaneous transplanted tumor

KW - Tissue fragment

KW - Transgenic mouse

UR - https://www.scopus.com/pages/publications/85067608191

U2 - 10.7652/jdyxb201903006

DO - 10.7652/jdyxb201903006

M3 - 文章

AN - SCOPUS:85067608191

SN - 1671-8259

VL - 40

SP - 368

EP - 373

JO - Journal of Xi'an Jiaotong University (Medical Sciences)

JF - Journal of Xi'an Jiaotong University (Medical Sciences)

IS - 3

ER -

转基因小鼠源性胰腺癌组织块皮下移植瘤模型的构建与评价

Abstract

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