等离子体活化气促进皮肤伤口愈合效应的实验研究

Objective To explore the effect of plasma-activated gas (PAG) in promoting wound healing. Methods The pro-healing effect of PAG was explored by testing the effectiveness of PAG in promoting cell proliferation and healing of infected wounds. Cell proliferation assay: five different cells (i.e.. HSF. IHSMC. WPMY-1. HaCaT. and HFF) in logarithmic growth phase were inoculated into culture plates. After the cells grew adherently to the wall, the different cells were each grouped experimentally using a suitable time gradient. After the cells were treated with PAG for different time, their activity was detected by CCK-8 method. Animal infected wound healing assay: a wound of about 1.5 cm X 1.5 cm in size was created on the back of SD rats with sterile tweezers and scissors, and then the wound was infected with P. Aeruginosa with A6oonm = 6.5. After the infection was completed, the wounds were treated with PAG at regular intervals, and the experiment groups were subdivided according to the different treatment durations. Wound healing photographs and changes in relative wound area were used as indicators of healing performance. Results Cell proliferation assay: PAG treatment was effective in promoting cell proliferation for a reasonable period of time, while overdose led to cell death. Therefore, the dose of cells treated with plasma activation gas was defined as= W/N (J/cell). where W is the work consumed by the activation gas device during the treatment process and N is the number of cells. The three indexes (starting dose, optima! dose and safe dose range) were used to characterize the proliferative effect of PAG. Animal infected wound healing assay: the experimental groups all showed accelerated wound healing, so the optimal treatment time was used to characterize the pro-healing effect of PAG. The sterilization mode played a primary role, with an optimal treatment time of 2 min. and the pro-healing mode played a secondary role. The treatment conditions with the best overall healing effect were 2 min for the sterilization mode and 1 min for the pro-healing mode. Conclusion PAG has the effect of promoting cell proliferation and infected wound healing, and indicators can be summarized to quantitatively describe its effect, which is conducive to further standardization of PAG research and clinical utility.