Abstract
Objective: To explore the inhibitory effect of resveratrol on hypertension-related myocardial fibrosis and the key role of TNF-β/Smads signaling pathway in the anti-fibrosis of resveratrol. Methods: The expression of vimentin in the primary rat CFs was evaluated by immunofluorescence to determine the cell type. CFs were treated in different conditions, the mRNA expression levels of type Ⅰ and type Ⅲ collagen and TGF-β1 were detected by Real-time PCR; the protein expression levels of type Ⅰ and type Ⅲ collagen, TGF-β1, Smad-3 and P-Smad-3 were detected by Western blot. The secretion levels of type Ⅰ collagen and TGF-β1 in CFs supernatant were measured by ELISA assay. Results: Rat CFs were successfully extracted, and vimentin expression was obvious. Real-time PCR results indicated that the gene expression levels of collagen Ⅰ and Ⅲ and TGF-β1 in CFs by exposure to AngⅡ were significantly increased as compared with those in normal group (P<0.05). However, AngⅡ-induced collagen Ⅰ and Ⅲ and TGF-β1 mRNA upregulation was inhibited by Res treatment (P<0.05). Western blot analysis showed that the protein expressions of collagen Ⅰ and Ⅲ and TGF-β1 in CFs were also increased after exposed to AngⅡ when compared to the normal controls (P<0.05). Similarly, AngⅡ-mediated collagen Ⅰ and Ⅲ and TGF-β1 upregulation was prevented by Res treatment (P<0.05). In addition, the phosphorylation level of Smad-3 was enhanced by both interventions (P<0.05). However, AngⅡ stimulated TGF-β1 upregulation while Smad-3 phosphorylation was suppressed by Res treatment (P<0.05). The secretion levels of collagen Ⅰ and TGF-β1 in CFs supernatant increased significantly in CFs exposed to AngⅡ condition as compared with those in normal condition (P<0.05). However, AngⅡ increased collagen Ⅰ and TGF-β1 secretion was prevented by Res intervention (P<0.05). Conclusion: Resveratrol inhibits the expressions of type Ⅰ and type Ⅲ collagen induced by AngⅡ in cardiac fibroblasts through regulating the TGF-β1/Smad3 signaling pathway.
| Translated title of the contribution | Resveratrol inhibits the expression of typeⅠ and type Ⅲ collagen induced by AngⅡ in cardiac fibroblasts through regulating the TGF-β1/Smad3 signaling pathway |
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| Original language | Chinese (Traditional) |
| Pages (from-to) | 525-529 and 557 |
| Journal | Journal of Xi'an Jiaotong University (Medical Sciences) |
| Volume | 39 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jul 2018 |
| Externally published | Yes |