牙周炎不同发展阶段牙周膜微环境中生化因素与力学因素的变化

Objective To investigate the patterns of changes in biochemical factors (IL-lß and TGF-ßl) and biophysical factors within the periodontal ligament microenvironment at different stages of Periodontitis development. Methods Periodontitis models at different stages of development (early and late stages) were construeted in rats. The changes in the periodontal ligament microenvironment were determined by detecting biochemical factors ((IL-lß and TGF-ßl) and changes in periodontal ligament stiffness. Results Regarding biochemical factors, the growth factor TGF-ßl was at a high level in the early stage of Periodontitis, while the inflammatory factor (IL-lß was slightly increased compared to the normal State, but expressed at a low level. As the inflammation progressed, the expression of TGF-ßl was suppressed and significantly decreased in the late stage of Periodontitis (latestage: 11.34 +2.91 vs. early stage; 20.41 +2.64, P<0.01). In contrast, the expression of (IL-lß significantly increased (late stage: 18.77 + 3.36 vs. early stage: 8.24+1.47, P<0.01). Regarding biophysical factors, the stiffness of the periodontal ligament and the expression of the key protein integrin a„ ß3 in the mechanical signaling pathway gradually decreased with the development of Periodontitis. Additionally, the osteogenic differentiation capacity of stem cells significantly decreased in the late stage of Periodontitis (late stage: 12.47 + 2.83 vi-. early stage: 21.15 + 4.38, P<0.05). Conclusion TGF-ßl may play a role in promoting periodontal tissue repair and maintaining physiological stability in normal and early stages of Periodontitis, while the increased expression of (IL-lß in the late stage of Periodontitis may inhibit the osteogenic differentiation of stem cells, leading to bone loss. Biophysical factors, including matrix stiffness and integrin avß3 expression, gradually decrease with the Progression of Periodontitis, potentially affecting bone tissue repair.