局部使用抗生素对拟行玻璃体内注射患者眼表微生物群落 影响的自身前后对照研究

Objective To investigate the effect of topical antibiotics on the microbiome of ocular surface in patients who received intravitreal injection. Methods Conjunctival sac swabs of patients who received intravitreal injection from September 2018 to January 2019,samples were taken before and after local antibiotic administration of the eyes (untreated group and treated group, respectively) to establish self-control study. MiseqPE301 + 8 + 8 + 301 platform was applied to carry double-terminal sequencing of bacterial 16S rDNA gene in V3 - V4 region, and compare the differences of microbial community composition in the two groups. OTUs clustering, species abundance analysis, diversity analysis, species composition classification analysis and other bioinformatics analysis were conducted. Results All the patients signed the informed consent. A total of 30 patients (43 eyes) and 86 samples were collected, and 41 samples were successfully sequenced, and the proper comparison was 7 patients (14 samples). During the detection, 607 362 valid sequences and 869 OTUs were obtained , respectively. OTUs analysis showed that the number of ocular surface microbe OTUs in the treated group was significantly lower than that in the untreated group. Both Alpha and Beta diversity analysis showed that there was no significant difference in ocular surface microbial communities between the treated group and the untreated group (both P > 0.05). Lefse analysis showed that there were significant differences in the core bacteria composition between the treated group and the untreated group. The untreated group was mainly actinomycetes, corynebacterium and staphylococcus epi-dermidis, while the treated group was mainly a-variant bacteria, spatholipidomonas and streptococcus. Conclusion Topical administration of antibiotics can significantly influence microbiome of ocular surface, especially some important " core bacteria" in the conjunctival sac which are important for maintaining ocular homeostasis.