基于叶绿素荧光成像技术的藻毒性检测法的建立及在环境监测中的应用

Using a novel large-area modulating chlorophyll fluorescence imaging system (Maxi-Imaging-PAM), a method for algae toxicity test was developed and optimized based on microplate. In this method, Chlorella pyrenoidosa was the testing organism, and typical photosynthesis inhibitors diuron and ametryn were chosen as testing substances. The influence of the experimental parameters on the results were discussed in this study. Finally, this developed method was applied on the algae toxicity test of petroleum wastewater. The results showed that when the concentration of the algal solution was 3.45×10⁶~5.28×10⁶ cells•mL^-1 and exposure time was 2 hours, the method displayed a good testing property. At this condition, the concentration of 50% maximal effect (EC₅₀) of diuron and ametryn were (4.33±0.38) μg•L^-1 and (3.87±0.27) μg•L^-1, respectively. The method was successfully applied to the algae toxicity test of petroleum wastewater, and its EC₅₀ value was 1.03%±0.07% (dilution percentage), which is equivalent to 414.96 μg•L^-1 diuron. The established microplate algae toxicity detection method has the advantages of high sensitivity, less sample volume demanded, and large batch test. This has great significance for the development of toxicology.