吸烟者外周血单个核细胞差异表达miRNA与mRNA调控网络

Objective: To explore the regulatory network of dysregulated microRNAs and mRNAs and related signaling pathways in peripheral mononuclear cells (PBMCs) of cigarette smokers. Methods: The peripheral venous blood was taken from 20 healthy non-smokers and 17 healthy smokers. The miRNA and mRNA expression profile of PBMCs was examined by miRNA and mRNA array. The regulatory relationship between dysregulated miRNAs and mRNAs was predicted and the signaling pathways were enriched. Results: Compared with the healthy non-smokers, the smokers had dysregulated miRNAs and mRNAs. The GO enrichment analysis indicated that PDGF signaling pathway, CCKR signaling map, T cell activation and EGF receptor signaling pathway were enriched in the smokers. Conclusion: The regulatory network of dysregulated miRNAs and mRNAs in PBMCs of smokers may be related to some potential risk factors for certain diseases.