凤庆滇红茶茶毫的化学成分

This paper investigates the chemical constituents of Yunnan black tea trichomes from Fengqing county. Using various chromatographic techniques including silica gel, Sephadex LH-20, C₁₈ reverse-phase, D101 macroporous adsorption resin column chromatography, and semi-preparative HPLC, ten compounds were isolated from the ethyl acetate fraction of a 95% ethanol extract of Yunnan black tea trichomes. Their structures were identified via spectroscopic methods (NMR, MS, and ECD) as: Caffeine (1), gallic acid (2), kaempferol (3), epicatechin (4), (+)-catechin (5), isoquercitrin (6), (−)-epicatechin-3-gallate (7), pyrrolidinone H (8), tiliroside (9), kaempferol-3-O [2, 6-di-O-(E)-p-coumaroyl]-β-D-glucopyranoside (10), respectively. Preliminary content analysis indicated a relatively high caffeine content (0.64% of dry weight), while tea polyphenols accounted for 0.03% of dry weight, suggesting Yunnan black tea trichomes warrants further development.