内环化聚(β-氨基酯)的制备与生物物理性能

The topological structure of polymers has a significant impact on their properties. In this study，we synthesized cyclized poly（β-amino ester）s by adjusting the polymerization parameters and investigated their physio⁃ logical properties. By utilizing trimethylolpropane triacrylate（TMPTA）and N-（3-Aminopropyl）morpholine（MPA）as monomers and varying their feed ratios and concentrations，two cyclized poly（β-amino ester）s were prepared through Michael addition reaction. The chemical composition，topological structure，and physiological properties of the cyclized poly（β-amino ester）s were characterized by gel permeation chromatography（GPC），nuclear magnetic reso⁃ nance spectroscopy（NMR），and atomic force microscopy（AFM）. The results showed that an increase in cyclization led to enhanced DNA condensation ability，faster degradation profiles，stronger proton buffering capacity，and higher cytotoxicity. These findings provide valuable insights into the preparation and characterization of cyclized poly（β-amino ester）s，shedding light on the design of reliable gene delivery vectors.